Cited 26 times in
Specific Detection of Influenza A and B Viruses by CRISPR-Cas12a-Based Assay
DC Field | Value | Language |
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dc.contributor.author | 이재면 | - |
dc.date.accessioned | 2021-09-29T01:19:35Z | - |
dc.date.available | 2021-09-29T01:19:35Z | - |
dc.date.issued | 2021-03 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/184310 | - |
dc.description.abstract | A rapid and accurate on-site diagnostic test for pathogens including influenza viruses is critical for preventing the spread of infectious diseases. Two types of influenza virus, A and B cause seasonal flu epidemics, whereas type A can cause influenza pandemics. To specifically detect influenza A (IAV) and B (IBV) viruses, we developed a clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR-associated (Cas) system-based assay. By coupling reverse transcription recombinase polymerase amplification (RT-RPA) and reverse transcription loop-mediated isothermal amplification (RT-LAMP), a CRISPR-Cas12a DNA endonuclease-targeted CRISPR trans-reporter (DETECTR) detected IAV and IBV titers as low as 1 × 100 plaque forming units (PFUs) per reaction without exhibiting cross-reactivity. Only 75 to 85 min were required to detect IAV and IBV, depending on isothermal nucleic acid amplification methods, and results were verified using a lateral flow strip assay that does not require additional analytic equipment. Taken together, our findings establish RT-RPA and RT-LAMP-coupled DETECTR-based diagnostic tests for rapid, specific and high-sensitivity detection of IAV and IBV using fluorescence and lateral flow assays. The diagnostic test developed in this study can be used to distinguish IAV and IBV infections, a capability that is necessary for monitoring and preventing the spread of influenza epidemics and pandemics. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | MDPI Pub. | - |
dc.relation.isPartOf | BIOSENSORS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | CRISPR-Cas Systems* | - |
dc.subject.MESH | Clustered Regularly Interspaced Short Palindromic Repeats | - |
dc.subject.MESH | Herpesvirus 1, Cercopithecine | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Influenza A virus / isolation & purification* | - |
dc.subject.MESH | Influenza B virus / isolation & purification* | - |
dc.subject.MESH | Influenza, Human / diagnosis | - |
dc.subject.MESH | Molecular Diagnostic Techniques | - |
dc.subject.MESH | Nucleic Acid Amplification Techniques | - |
dc.subject.MESH | Pandemics | - |
dc.subject.MESH | Reverse Transcription | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.title | Specific Detection of Influenza A and B Viruses by CRISPR-Cas12a-Based Assay | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Bum Ju Park | - |
dc.contributor.googleauthor | Man Seong Park | - |
dc.contributor.googleauthor | Jae Myun Lee | - |
dc.contributor.googleauthor | Yoon Jae Song | - |
dc.identifier.doi | 10.3390/bios11030088 | - |
dc.contributor.localId | A03071 | - |
dc.relation.journalcode | J04064 | - |
dc.identifier.eissn | 2079-6374 | - |
dc.identifier.pmid | 33808752 | - |
dc.subject.keyword | CRISPR-Cas12a | - |
dc.subject.keyword | DETECTR | - |
dc.subject.keyword | diagnosis | - |
dc.subject.keyword | influenza virus | - |
dc.contributor.alternativeName | Lee, Jae Myun | - |
dc.contributor.affiliatedAuthor | 이재면 | - |
dc.citation.volume | 11 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 88 | - |
dc.identifier.bibliographicCitation | BIOSENSORS, Vol.11(3) : 88, 2021-03 | - |
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