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Retroductal Delivery of Epidermal Growth Factor Protects Salivary Progenitors after Irradiation

Authors
 J M Cho  ;  Y J Yoon  ;  S Lee  ;  D Kim  ;  D Choi  ;  J Kim  ;  J Y Lim 
Citation
 JOURNAL OF DENTAL RESEARCH, Vol.100(8) : 883-890, 2021-07 
Journal Title
JOURNAL OF DENTAL RESEARCH
ISSN
 0022-0345 
Issue Date
2021-07
Keywords
apoptosis ; organoid ; progenitor ; salivary ducts ; salivary glands ; xerostomia
Abstract
Salivary gland hypofunction after irradiation is associated with a deficit of epithelial stem/progenitors in salivary glands. Although epidermal growth factor (EGF) is known to stimulate the proliferation of epithelial cells, the therapeutic effect of EGF on salivary epithelial stem/progenitors remains undetermined. In this study, we administered EGF to submandibular glands (SMGs) via a retrograde route through the SMG excretory duct before fractionated irradiation and examined whether EGF could protect salivary epithelial progenitor cells from radiation and alleviate radiation-induced salivary hypofunction. EGF-treated mice exhibited greater body and gland weights at 12 wk after irradiation than untreated mice. The retroductal delivery of EGF improved salivary secretory function and increased salivary amylase activity in a dose-dependent manner. Histological examinations highlighted the amelioration of the loss of keratine-14+ (KRT14+) basal ductal and/or MIST1+ acinar cells, as well as induction of fibrosis, following irradiation in EGF-treated mice. An additional in vitro experiment using a salivary gland organoid irradiation model indicated that the radioprotective effects of EGF promoted the growth and inhibited the apoptotic cell death of salivary epithelial cells. Our results suggest that retroductal delivery of EGF may be a promising therapeutic option for preventing radiation-induced salivary gland hypofunction.
Full Text
https://journals.sagepub.com/doi/10.1177/0022034521999298
DOI
10.1177/0022034521999298
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Donghyun(김동현) ORCID logo https://orcid.org/0000-0001-7863-7384
Yoon, Y J(윤여준)
Lim, Jae Yol(임재열) ORCID logo https://orcid.org/0000-0002-9757-6414
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184218
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