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Plasmon-enhanced fluorescence correlation spectroscopy for super-localized detection of nanoscale subcellular dynamics

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dc.contributor.author신전수-
dc.contributor.author이우중-
dc.contributor.author이우중-
dc.date.accessioned2021-09-29T01:04:29Z-
dc.date.available2021-09-29T01:04:29Z-
dc.date.issued2021-07-
dc.identifier.issn0956-5663-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/184178-
dc.description.abstractIn this report, we investigate plasmon-enhanced imaging fluorescence correlation spectroscopy (p-FCS). p-FCS takes advantage of extreme light confinement by localization at nanogap-based plasmonic nanodimer arrays (PNAs) for enhanced signal-to-noise ratio (SNR) and improved precision by registration with surface plasmon microscopy images. Theoretical results corroborate the enhancement by PNAs in the far-field. Near-field scanning optical microscopy was used to confirm near-field localization experimentally. Experimental confirmation was also conducted with fluorescent nanobeads. The concept was further applied to studying the diffusion dynamics of lysosomes in HEK293T cells stimulated by phorbol 12-myristate 13-acetate treatment. It was found that lysosomes demonstrate stronger super-diffusive behavior with relatively weaker sub-diffusion after stimulation. SNR measured of p-FCS was improved by 9.77 times over conventional FCS. This report is expected to serve as the foundation for an enhanced analytical tool to explore subcellular dynamics.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier Advanced Technology-
dc.relation.isPartOfBIOSENSORS & BIOELECTRONICS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHBiosensing Techniques*-
dc.subject.MESHDiffusion-
dc.subject.MESHHEK293 Cells-
dc.subject.MESHHumans-
dc.subject.MESHMicroscopy, Fluorescence-
dc.subject.MESHSpectrometry, Fluorescence-
dc.titlePlasmon-enhanced fluorescence correlation spectroscopy for super-localized detection of nanoscale subcellular dynamics-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학교실)-
dc.contributor.googleauthorHongki Lee-
dc.contributor.googleauthorWoo Joong Rhee-
dc.contributor.googleauthorGwiyeong Moon-
dc.contributor.googleauthorSeongmin Im-
dc.contributor.googleauthorTaehwang Son-
dc.contributor.googleauthorJeon-Soo Shin-
dc.contributor.googleauthorDonghyun Kim-
dc.identifier.doi10.1016/j.bios.2021.113219-
dc.contributor.localIdA02144-
dc.contributor.localIdA05006-
dc.contributor.localIdA05006-
dc.relation.journalcodeJ00330-
dc.identifier.eissn1873-4235-
dc.identifier.pmid33895690-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0956566321002566-
dc.subject.keywordFluorescence correlation spectroscopy-
dc.subject.keywordLysosome-
dc.subject.keywordNanogap-
dc.subject.keywordPlasmonic nanodimer arrays-
dc.subject.keywordPlasmonics-
dc.subject.keywordSuper-localization-
dc.contributor.alternativeNameShin, Jeon Soo-
dc.contributor.affiliatedAuthor신전수-
dc.contributor.affiliatedAuthor이우중-
dc.contributor.affiliatedAuthor이우중-
dc.citation.volume184-
dc.citation.startPage113219-
dc.identifier.bibliographicCitationBIOSENSORS & BIOELECTRONICS, Vol.184 : 113219, 2021-07-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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