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Downregulation of MicroRNA-495 Alleviates IL-1β Responses among Chondrocytes by Preventing SOX9 Reduction

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dc.contributor.author김성환-
dc.contributor.author박광환-
dc.contributor.author이경미-
dc.contributor.author이진우-
dc.contributor.author박광환-
dc.contributor.author이경미-
dc.contributor.author이진우-
dc.contributor.author윤동석-
dc.date.accessioned2021-09-29T01:01:40Z-
dc.date.available2021-09-29T01:01:40Z-
dc.date.issued2021-07-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/184151-
dc.description.abstractPurpose: Our previous work demonstrated that miRNA-495 targets SOX9 to inhibit chondrogenesis of mesenchymal stem cells. In this study, we aimed to investigate whether miRNA-495-mediated SOX9 regulation could be a novel therapeutic target for osteoarthritis (OA) using an in vitro cell culture model. Materials and methods: An in vitro model mimicking the OA environment was established using TC28a2 normal human chondrocyte cells. Interleukin-1β (IL-1β, 10 ng/mL) was utilized to induce inflammation-related changes in TC28a2 cells. Safranin O staining and glycosaminoglycan assay were used to detect changes in proteoglycans among TC28a2 cells. Expression levels of COX-2, ADAMTS5, MMP13, SOX9, CCL4, and COL2A1 were examined by qRT-PCR and/or Western blotting. Immunohistochemistry was performed to detect SOX9 and CCL4 proteins in human cartilage tissues obtained from patients with OA. Results: miRNA-495 was upregulated in IL-1β-treated TC28a2 cells and chondrocytes from damaged cartilage tissues of patients with OA. Anti-miR-495 abolished the effect of IL-1β in TC28a2 cells and rescued the protein levels of SOX9 and COL2A1, which were reduced by IL-1β. SOX9 was downregulated in the damaged cartilage tissues of patients with OA, and knockdown of SOX9 abolished the effect of anti-miR-495 on IL-1β-treated TC28a2 cells. Conclusion: We demonstrated that inhibition of miRNA-495 alleviates IL-1β-induced inflammatory responses in chondrocytes by rescuing SOX9 expression. Accordingly, miRNA-495 could be a potential novel target for OA therapy, and the application of anti-miR-495 to chondrocytes could be a therapeutic strategy for treating OA.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChondrocytes* / metabolism-
dc.subject.MESHDown-Regulation-
dc.subject.MESHHumans-
dc.subject.MESHInterleukin-1beta* / metabolism-
dc.subject.MESHMicroRNAs* / genetics-
dc.subject.MESHSOX9 Transcription Factor* / genetics-
dc.subject.MESHSOX9 Transcription Factor* / metabolism-
dc.titleDownregulation of MicroRNA-495 Alleviates IL-1β Responses among Chondrocytes by Preventing SOX9 Reduction-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Orthopedic Surgery (정형외과학교실)-
dc.contributor.googleauthorSoyeong Joung-
dc.contributor.googleauthorDong Suk Yoon-
dc.contributor.googleauthorSehee Cho-
dc.contributor.googleauthorEun Ae Ko-
dc.contributor.googleauthorKyoung Mi Lee-
dc.contributor.googleauthorKwang Hwan Park-
dc.contributor.googleauthorJin Woo Lee-
dc.contributor.googleauthorSung Hwan Kim-
dc.identifier.doi10.3349/ymj.2021.62.7.650-
dc.contributor.localIdA00592-
dc.contributor.localIdA01437-
dc.contributor.localIdA04619-
dc.contributor.localIdA03230-
dc.contributor.localIdA01437-
dc.contributor.localIdA04619-
dc.contributor.localIdA03230-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid34164963-
dc.subject.keywordChondrocytes-
dc.subject.keywordSOX9-
dc.subject.keywordinflammation-
dc.subject.keywordmiRNA-495-
dc.subject.keywordosteoarthritis-
dc.contributor.alternativeNameKim, Sung Hwan-
dc.contributor.affiliatedAuthor김성환-
dc.contributor.affiliatedAuthor박광환-
dc.contributor.affiliatedAuthor이경미-
dc.contributor.affiliatedAuthor이진우-
dc.contributor.affiliatedAuthor박광환-
dc.contributor.affiliatedAuthor이경미-
dc.contributor.affiliatedAuthor이진우-
dc.citation.volume62-
dc.citation.number7-
dc.citation.startPage650-
dc.citation.endPage659-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.62(7) : 650-659, 2021-07-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers

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