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Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway

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dc.contributor.author정한성-
dc.contributor.author김현이-
dc.date.accessioned2021-09-29T00:44:08Z-
dc.date.available2021-09-29T00:44:08Z-
dc.date.issued2021-05-
dc.identifier.issn1350-9047-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/184008-
dc.description.abstractSpermatogenesis is an important cellular differentiation process that produces the male gametes and remains active throughout the individual's lifespan. Sertoli cell-only syndrome (SCO) refers to the dysfunction of the male reproductive system, including infertility. Accurate self-renewal of spermatogonial stem cells (SSCs) is essential to prevent SCO syndrome. This study investigated the role of microtubule-associated serine/threonine kinase family member 4 (MAST4) in spermatogenesis in mice. MAST4 was localized in Sertoli cells before puberty, providing a somatic niche for spermatogenesis in mice and MAST4 expression shifted to Leydig cells and spermatids throughout puberty. Mast4 knockout (KO) testes were reduced in size compared to wild-type testes, and germ cell depletion associated with an increase in apoptosis and subsequent loss of tubular structure were similar to the SCO phenotype. In addition, MAST4 phosphorylated the Ets-related molecule (ERM), specifically the serine 367 residue. The phosphorylation of ERM ultimately controls the transcription of ERM target genes related to SSC self-renewal. The expression of spermatogenesis-associated proteins was significantly decreased whereas Sertoli cell markers were increased in Mast4 KO testes, which was well-founded by RNA-sequencing analysis. Therefore, MAST4 is associated with the fibroblast growth factor 2 (FGF2)/ERM pathway and this association helps us explore the capacity of SSCs to maintain a vertebrate stem cell niche.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherNature Publishing Group-
dc.relation.isPartOfCELL DEATH AND DIFFERENTIATION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleMast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학교실)-
dc.contributor.googleauthorSeung-Jun Lee-
dc.contributor.googleauthorJinah Park-
dc.contributor.googleauthorDong-Joon Lee-
dc.contributor.googleauthorKeishi Otsu-
dc.contributor.googleauthorPyunggang Kim-
dc.contributor.googleauthorSeiya Mizuno-
dc.contributor.googleauthorMin-Jung Lee-
dc.contributor.googleauthorHyun-Yi Kim-
dc.contributor.googleauthorHidemitsu Harada-
dc.contributor.googleauthorSatoru Takahashi-
dc.contributor.googleauthorSeong-Jin Kim-
dc.contributor.googleauthorHan-Sung Jung-
dc.identifier.doi10.1038/s41418-020-00670-2-
dc.contributor.localIdA03758-
dc.relation.journalcodeJ00483-
dc.identifier.eissn1476-5403-
dc.identifier.pmid33219327-
dc.identifier.urlhttps://www.nature.com/articles/s41418-020-00670-2-
dc.contributor.alternativeNameJung, Han Sung-
dc.contributor.affiliatedAuthor정한성-
dc.citation.volume28-
dc.citation.number5-
dc.citation.startPage1441-
dc.citation.endPage1454-
dc.identifier.bibliographicCitationCELL DEATH AND DIFFERENTIATION, Vol.28(5) : 1441-1454, 2021-05-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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