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Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway
DC Field | Value | Language |
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dc.contributor.author | 정한성 | - |
dc.contributor.author | 김현이 | - |
dc.date.accessioned | 2021-09-29T00:44:08Z | - |
dc.date.available | 2021-09-29T00:44:08Z | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 1350-9047 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/184008 | - |
dc.description.abstract | Spermatogenesis is an important cellular differentiation process that produces the male gametes and remains active throughout the individual's lifespan. Sertoli cell-only syndrome (SCO) refers to the dysfunction of the male reproductive system, including infertility. Accurate self-renewal of spermatogonial stem cells (SSCs) is essential to prevent SCO syndrome. This study investigated the role of microtubule-associated serine/threonine kinase family member 4 (MAST4) in spermatogenesis in mice. MAST4 was localized in Sertoli cells before puberty, providing a somatic niche for spermatogenesis in mice and MAST4 expression shifted to Leydig cells and spermatids throughout puberty. Mast4 knockout (KO) testes were reduced in size compared to wild-type testes, and germ cell depletion associated with an increase in apoptosis and subsequent loss of tubular structure were similar to the SCO phenotype. In addition, MAST4 phosphorylated the Ets-related molecule (ERM), specifically the serine 367 residue. The phosphorylation of ERM ultimately controls the transcription of ERM target genes related to SSC self-renewal. The expression of spermatogenesis-associated proteins was significantly decreased whereas Sertoli cell markers were increased in Mast4 KO testes, which was well-founded by RNA-sequencing analysis. Therefore, MAST4 is associated with the fibroblast growth factor 2 (FGF2)/ERM pathway and this association helps us explore the capacity of SSCs to maintain a vertebrate stem cell niche. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | CELL DEATH AND DIFFERENTIATION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학교실) | - |
dc.contributor.googleauthor | Seung-Jun Lee | - |
dc.contributor.googleauthor | Jinah Park | - |
dc.contributor.googleauthor | Dong-Joon Lee | - |
dc.contributor.googleauthor | Keishi Otsu | - |
dc.contributor.googleauthor | Pyunggang Kim | - |
dc.contributor.googleauthor | Seiya Mizuno | - |
dc.contributor.googleauthor | Min-Jung Lee | - |
dc.contributor.googleauthor | Hyun-Yi Kim | - |
dc.contributor.googleauthor | Hidemitsu Harada | - |
dc.contributor.googleauthor | Satoru Takahashi | - |
dc.contributor.googleauthor | Seong-Jin Kim | - |
dc.contributor.googleauthor | Han-Sung Jung | - |
dc.identifier.doi | 10.1038/s41418-020-00670-2 | - |
dc.contributor.localId | A03758 | - |
dc.relation.journalcode | J00483 | - |
dc.identifier.eissn | 1476-5403 | - |
dc.identifier.pmid | 33219327 | - |
dc.identifier.url | https://www.nature.com/articles/s41418-020-00670-2 | - |
dc.contributor.alternativeName | Jung, Han Sung | - |
dc.contributor.affiliatedAuthor | 정한성 | - |
dc.citation.volume | 28 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1441 | - |
dc.citation.endPage | 1454 | - |
dc.identifier.bibliographicCitation | CELL DEATH AND DIFFERENTIATION, Vol.28(5) : 1441-1454, 2021-05 | - |
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