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Immunization with RBD-P2 and N protects against SARS-CoV-2 in nonhuman primates

Authors
 So-Hee Hong  ;  Hanseul Oh  ;  Yong Wook Park  ;  Hye Won Kwak  ;  Eun Young Oh  ;  Hyo-Jung Park  ;  Kyung Won Kang  ;  Green Kim  ;  Bon-Sang Koo  ;  Eun-Ha Hwang  ;  Seung Ho Baek  ;  Hyeong-Jun Park  ;  Yu-Sun Lee  ;  Yoo-Jin Bang  ;  Jae-Yong Kim  ;  Seo-Hyeon Bae  ;  Su Jeen Lee  ;  Ki-Weon Seo  ;  Hak Kim  ;  Taewoo Kwon  ;  Ji-Hwan Kim  ;  Seonghwan Lee  ;  Eunsom Kim  ;  Yeonhwa Kim  ;  Jae-Hak Park  ;  Sang-In Park  ;  Marta Gonçalves  ;  Byung Mook Weon  ;  Haengdueng Jeong  ;  Ki Taek Nam  ;  Kyung-Ah Hwang  ;  Jihye Kim  ;  Hun Kim  ;  Sang-Myeong Lee  ;  Jung Joo Hong  ;  Jae-Hwan Nam 
Citation
 SCIENCE ADVANCES, Vol.7(22) : eabg7156, 2021-05 
Journal Title
SCIENCE ADVANCES
Issue Date
2021-05
MeSH
Animals ; COVID-19 / genetics ; COVID-19 / immunology ; COVID-19 / prevention & control* ; COVID-19 Vaccines / genetics ; COVID-19 Vaccines / immunology* ; Chlorocebus aethiops ; Coronavirus Nucleocapsid Proteins / genetics ; Coronavirus Nucleocapsid Proteins / immunology* ; Female ; Macaca fascicularis ; Mice ; Mice, Inbred BALB C ; Mice, Transgenic ; Phosphoproteins / genetics ; Phosphoproteins / immunology ; Protein Domains ; Rats ; Recombinant Fusion Proteins / genetics ; Recombinant Fusion Proteins / immunology* ; SARS-CoV-2 / genetics ; SARS-CoV-2 / immunology* ; Sf9 Cells ; Spike Glycoprotein, Coronavirus / genetics ; Spike Glycoprotein, Coronavirus / immunology* ; Spodoptera ; Tetanus Toxoid / genetics ; Tetanus Toxoid / immunology* ; Vero Cells
Abstract
Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), various vaccines are being developed, with most vaccine candidates focusing on the viral spike protein. Here, we developed a previously unknown subunit vaccine comprising the receptor binding domain (RBD) of the spike protein fused with the tetanus toxoid epitope P2 (RBD-P2) and tested its efficacy in rodents and nonhuman primates (NHPs). We also investigated whether the SARS-CoV-2 nucleocapsid protein (N) could increase vaccine efficacy. Immunization with N and RBD-P2 (RBDP2/N) + alum increased T cell responses in mice and neutralizing antibody levels in rats compared with those obtained using RBD-P2 + alum. Furthermore, in NHPs, RBD-P2/N + alum induced slightly faster SARS-CoV-2 clearance than that induced by RBD-P2 + alum, albeit without statistical significance. Our study supports further development of RBD-P2 as a vaccine candidate against SARS-CoV-2. Also, it provides insights regarding the use of N in protein-based vaccines against SARS-CoV-2.
Files in This Item:
T202101849.pdf Download
DOI
10.1126/sciadv.abg7156
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Nam, Ki Taek(남기택)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/183998
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