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Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR
- Authors
- So Yeon Kim ; Jaehyeon Lee ; Heungsup Sung ; Hyukmin Lee ; Myung Guk Han ; Cheon Kwon Yoo ; Sang Won Lee ; Ki Ho Hong
- Citation
- EMERGING INFECTIOUS DISEASES, Vol.26(10) : 2469-2472, 2020-10
- Journal Title
- EMERGING INFECTIOUS DISEASES
- ISSN
- 1080-6040
- Issue Date
- 2020-10
- MeSH
- Betacoronavirus / isolation & purification* ; COVID-19 ; COVID-19 Testing ; COVID-19 Vaccines ; Clinical Laboratory Techniques / methods* ; Coronavirus Infections / diagnosis* ; Humans ; Mass Screening / methods* ; Nasopharynx / virology ; Oropharynx / virology ; Pandemics ; Pneumonia, Viral / diagnosis* ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2 ; Sensitivity and Specificity
- Keywords
- COVID-19 ; SAR-CoV-2 ; SARS ; SARS-CoV-2 ; SARS-related coronavirus ; South Korea ; coronavirus disease ; real-time PCR ; respiratory infections ; reverse transcription PCR ; severe acute respiratory syndrome ; severe acute respiratory syndrome coronavirus 2 ; specimen pooling ; viruses ; zoonoses
- Abstract
- To validate the specimen-pooling strategy for real-time reverse transcription PCR detection of severe acute respiratory syndrome coronavirus 2, we generated different pools including positive specimens, reflecting the distribution of cycle threshold values at initial diagnosis. Cumulative sensitivities of tested pool sizes suggest pooling of <6 specimens for surveillance by this method.
- Files in This Item:
- T202007258.pdf Download
- Appears in Collections:
- 1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
- Yonsei Authors
-
Lee, Hyuk Min(이혁민)
https://orcid.org/0000-0002-8523-4126
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