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TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays

Authors
 Layla Panahipour  ;  Zahra Kargarpour  ;  Bernadette Luza  ;  Jung-Seok Lee  ;  Reinhard Gruber 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.21(18) : 6636, 2020-09 
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN
 1661-6596 
Issue Date
2020-09
MeSH
Collagen* ; Collagenases ; Dentistry ; Membranes, Artificial* ; Regenerative Medicine ; Transforming Growth Factor beta*
Keywords
TGF-β activity ; collagen membranes ; guided bone regeneration ; in vitro
Abstract
Collagen membranes commonly used in guided bone regeneration are supposed to actively influence tissue regeneration and are not exclusively serving as passive barriers shielding away the soft tissue. The molecular mechanisms by which collagen membranes might affect tissue regeneration might involve the activation of transforming growth factor beta (TGF-β) signaling pathways. Here, we determined the TGF-β activity of supernatants and proteolytic lysates of five commercially available collagen membranes. The expression of TGF-β target genes interleukin 11 (IL11), NADPH oxidase 4 (NOX4), and proteoglycan 4 (PRG4) was evaluated by reverse transcriptase polymerase chain reaction and IL11 immunoassay in gingival fibroblasts. TGF-β signaling activation was further assessed by blocking the TGF-β receptor I kinase, a TGF-β neutralizing antibody, and showing the nuclear localization of phosphorylated Smad3 and total Smad2/3. We could identify two collagen membranes whose supernatants and lysates caused a robust increase of TGF-β receptor I kinase-dependent expression of IL11 in gingival fibroblasts. Moreover, the supernatant of a particular one membrane caused the nuclear localization of phosphorylated Smad3 and Smad2/3 in the fibroblasts. These results strengthen the evidence that some collagen membranes possess an intrinsic TGF-β activity that might actively influence the process of guided bone regeneration.
Files in This Item:
T202007170.pdf Download
DOI
10.3390/ijms21186636
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Jung Seok(이중석) ORCID logo https://orcid.org/0000-0003-1276-5978
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/183858
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