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우리 나라에서 분리한 이질아메바 (Ebtanieba gustikttuca)와 동형아메바(Entamoeba dispar)의 감별

Other Titles
 Differentiation of Korean isolates of Entamoeba histolytica from Entamoeba dispar 
Authors
 최성준  ;  이미정  ;  이상금  ;  임경일  ;  이순형  ;  홍성태 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.34(1) : 15-20, 1996-03 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
1996-03
Abstract
Cysts of Entamoeba histolytica are still found from humans in Korea, but not all of the cysts are known as pathogenic. The non-pathogenic strain is regarded as a different species, E. dispar. In this study, Korean isolates of conventional E. histolytica were subjected for the differentiation by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. Human stools were screened by routine microscopic examination, and cyst or trophozoite positive stools were inoculated into Robinson media. The cultivated trophozoite positive stools were inoculated into Robinson media. The cultivated trophozoites were prepared for DNA extraction, and the DNAs were used for PCR with common primers of P1 gene. The PCR products were digested with 3 restriction enzymes and RFLP was observed. Also anti- sense primers containing the cleavage site of each restriction enzyme were designed for differentiation only by PCR. The PCR products of Korean isolates S9, S12, YS-6, and YS-27 were spliced by Taq I and Xmn I but not by Acc I, and the isolates S1, S3, S11, S15, S16, S17, S20, YS-17, and YS-44 were spliced by Acc I but not by Taq I and Xmn I. These RFLP pattern correlated well with PCR products by the species specific primers. The findings confirm that the Korean isolates S9, S12, YS-6, and YS-27 are E. histolytica and others are E. dispar. In Korea, most of the asymptomatic cyst carriers are infected by E. dispar, not by E. histolytica.
Files in This Item:
T199602273.pdf Download
DOI
10.3347/kjp.1996.34.1.15
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Im, Kyung Il(임경일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/183626
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