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사람 비강점막 혈관내피세포의 렉틴 결합반응

Other Titles
 Lectin-binding Patterns with Endothelial Cells of Human Nasal Mucosa 
Authors
 김경수  ;  이정권  ;  윤주헌  ;  박인용  ;  김명상  ;  최재진 
Citation
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.39(6) : 957-964, 1996-06 
Journal Title
Korean Journal of Otolaryngology(대한이비인후과학회지)
ISSN
 1225-035X 
Issue Date
1996-06
Keywords
Lectin ; Human nasal mucosa ; Endothelium ; ABO blood group ; Marker
Abstract
Lectin-binding patterns were observed with endothelial cells of human nasal mucosa according to the ABO blood group and they were obseved to find the marker lectin differentiating fenstrated from continuous capillary. Mucosal specimens were obtained from the inferior turbinate in 85 patients who underwent the intranasal ethmoidectomy or septoplasty. All specimens were fixed in 10% buffered formalin and embedded in paraffin. Serial sections, 4 μm in thickness, were made and stained with blood group specific lectins(DBA, GSI-B4, UEA-I) and blood group non-specific lectins(SBA, PNA, RCA-I, WGA, Con-A) using avidin-biotin peroxidase method. Diaminobenzidine was used as a chromogen. The sections were counterstained with Mayer's Hematoxylin. Lectin-binding patterns were observed using Vanox-S light microscope. UEA-I, WGA, RCA-I, and Con-A were labelled irrespective of blood group. UEA-I reaction was positive in capillary of deep portion, venule, and sinusoid. But no reaction was noted in capillary near gland. These results suggest that lectin-binding patterns with endothelial cell of human nasal mucosa in non-specific to blood group and UEA-I can be used as marker lectin differentiating fenestrated and continuous capillaries. Further study using epon-embedding method and immunoelectromicrocsopy will be required.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/183448
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