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뇌허혈 재관류로 인한 뇌조직 아민 변동과 nitric oxide 및 산소유리기와의 관련성
DC Field | Value | Language |
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dc.contributor.author | 김동구 | - |
dc.date.accessioned | 2021-09-28T08:07:01Z | - |
dc.date.available | 2021-09-28T08:07:01Z | - |
dc.date.issued | 1995-12 | - |
dc.identifier.issn | 1225-7044 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/183424 | - |
dc.description.abstract | Recently oxygen free radicals and nitric oxide (NO) are known to play an important role in neuronal reperfusion injury. This study was aimed to investigate the role of oxygen f ree radicals and NO during cerebral ischemia/reperfusion, using dimethylthiourea (DMTU) and NG-monomethyl-L-arginine (NMMA), an oxygen f ree radical scavenger and a competitive NOS inhibitor respectively. In the in vivo experiment, the ischemia/reperfusion-induced changes of cerebral biogenic amines were examined in Mongolian gerbil (Meriones unguiculatus) pre-treated with NMMA and/or DMTU. To induce cerebral ischemia/reperfusion, bilateral common carotid arteries were clamped for 10 minutes and then released for 15 minutes. The biogenic amines were measured by using HPLC-ECD(High Performance Liquid Chromatography-Electrochemical detection). To confirm the results from the in vivo experiments, the effect of NMMA and/or DMTU on [3H]dopamine release from striatal slices exposed to hypoxia was investigated. The results are as follows; 1) Ischemia/reperfusion increased the ratio of DOPAC/dopamine and HVA/dopamine as well as the concentrations of DOPAC and HVA, which were evident only in corpus striatum. 2) NMMA attenuated the ischemia/reperfusion-induced increase in the ratio of DOPAC/dopamine in corpus striatum. However, the change of DOPAC or HVA was minimal. 3) DMTU attenuated the ischemia/reperfusion-induced increase of DOPAC and HVA, and the ratio ofDOPAC / dopa- mine and HVA/dopamine in corpus striatum. 4) Simultaneous pre-treatment with NMMA and DMTU attenuated the ischemia/reperfusion-induced increase of DOPAC and HVA, and the ratio Of DOPAC/dopamine and HVA/dopamine in corpus striatum. The extent of attenuation was greater than the single treatment group with NMMA or DMTU. 5) Exposure to hypoxia markedly increased the release of [3H]dopamine in the striatal slices. 6) The administration of either NMMA or DMTU attenuated the increase of [3H]dopamine release induced by hypoxia in the striatal slices. 7) The administration of both NMMA or DMTU markedly attenuated the increase of [3H]dopamine release induced by hypoxia to the extent of the control in the striatal slices. These results suggest that oxygen free radicals play an important role in cerebral ischemia/reperfusion injury, for which NO seems to be responsible. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 대한신경과학회 | - |
dc.relation.isPartOf | Journal of the Korean Neurological Association(대한신경과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | 뇌허혈 재관류로 인한 뇌조직 아민 변동과 nitric oxide 및 산소유리기와의 관련성 | - |
dc.title.alternative | Relationship of the Changes in Biogenic Amines to Nitric Oxide and Oxygen Free Radicals Durign Cerebral Ishemia/Reperfusion | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학교실) | - |
dc.contributor.googleauthor | 문준식 | - |
dc.contributor.googleauthor | 정희선 | - |
dc.contributor.googleauthor | 김동구 | - |
dc.contributor.googleauthor | 김경환 | - |
dc.contributor.googleauthor | 이병철 | - |
dc.contributor.localId | A00396 | - |
dc.relation.journalcode | J01836 | - |
dc.subject.keyword | Ischemia | - |
dc.subject.keyword | Reperfusion | - |
dc.subject.keyword | Biogenic amine | - |
dc.subject.keyword | Dopamine | - |
dc.subject.keyword | DMTU | - |
dc.subject.keyword | NMMA | - |
dc.contributor.alternativeName | Kim, Dong Goo | - |
dc.contributor.affiliatedAuthor | 김동구 | - |
dc.citation.volume | 13 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 773 | - |
dc.citation.endPage | 787 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Neurological Association (대한신경과학회지), Vol.13(4) : 773-787, 1995-12 | - |
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