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Polymerase chain reaction reveals herpes simplex virus DNA in saliva of patients with Behçet’s disease

Authors
 Sungnack Lee  ;  Dongsik Bang  ;  Young Ho Cho  ;  Eun-So Lee  ;  Seonghyang Sohn 
Citation
 ARCHIVES OF DERMATOLOGICAL RESEARCH, Vol.288(4) : 179-183, 1996-01 
Journal Title
ARCHIVES OF DERMATOLOGICAL RESEARCH
ISSN
 0340-3696 
Issue Date
1996-01
MeSH
Base Sequence ; Behcet Syndrome / complications ; Behcet Syndrome / virology* ; DNA, Viral / analysis* ; DNA, Viral / genetics ; Humans ; Molecular Sequence Data ; Mouth Diseases / complications ; Mouth Diseases / virology ; Oligonucleotide Probes / genetics ; Polymerase Chain Reaction ; Reference Values ; Saliva / microbiology* ; Simplexvirus / genetics* ; Ulcer / complications ; Ulcer / virology
Abstract
The etiology of Behçet’s disease is unclear, but viral infection is thought to be one etiologic factor. The aims of this study were to detect herpes simplex virus (HSV) DNA in the saliva of patients with Behçet’s disease and of healthy persons, to determine whether the presence of HSV in saliva is associated with the presence of intraoral ulcer, and to investigate the relationship between HSV and Behçet’s disease. The polymerase chain reaction (PCR) was used to detect HSV DNA sequences in DNA extracted from the saliva of patients with Behçet’s disease and of healthy control subjects. Of 66 patients with Behçet’s disease diagnosed clinically, 19 were diagnosed as complete type, 29 as incomplete type and 18 as suspected type. Of 66 DNA preparations from the saliva of the patients, 26 (39.4%) showed the 289-bp band. This contrasts with 12 of 87 preparations (13.8%) from healthy controls (P<0.01). There were no, significant differences among the three patient groups. All the 289-bp bands analyzed by restriction endonuclease digestion yielded the expected 158-bp and 131-bp fragments when digested withPst I. HSV DNA was detected in 12 of 33 Behçet’s disease patients (36.4%) with oral ulceration and 14 of 33 patients (42.4%) without oral ulceration at the time of testing. There was no statistically significant correlation in the PCR results between the two groups.
Full Text
http://link.springer.com/article/10.1007%2FBF02505221
DOI
10.1007/BF02505221
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Bang, Dong Sik(방동식)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/183311
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