소사체 치아에서의 유전자지문 분석을 위한 실험적 연구

Abstract

In the field of inidvidual identification in forensic science, if the body is charred, it is sometimes impossible to identify the morphologic changes and charred tissue such as blood, muscle and bone can not be identified by forensic nicrobiologic method such as DNA typing. So the author used the characteristics of teeth which is relatively firm compare to other organs and stable to external environment such as heat and also possess cells needed for the DNA typing. The author conducted the experiment on teeth to detect DNA related to indivich,al identification regarding to temperature in which other charred organs can not be detected. The experiment was done on 64 extracted third.molars consisted of-unheated ones, and heated .teeth to´100´C,.150t, 200.0 for 45 min, 90 min, and 120 min respectively and to 250c for 45 min. DNA was extracted from each tooth and amplified- fragment length polymorphism procedure(AMP-FLPs) using polymerase chain reaction(PCR) was applied and observed for the matching DNA in HumTH01 and HuinCD4 locus and the followings are the results

1. It was able to detect matching DNA in HumTH01 and HumCD4 locus in every teeth which no heating has been done. 2. It was able to detect matching DNA in HumTH01 and HumCD4 locus in every teeth heated to 1001C for 45, 90 and 120 min. 3. It was able to detect matching DNA in HumTH01 and HumCD4 locus in teeth heated to 150C, 200C for 45, 90, 120 min. 4. It was impossible to detect matching DNA in HumTH01 and HumCD4 locus in teeth heated to 2500.

So, it is possible to extract DNA from teeth that otherwise can not be extracted from other organs in the charred body and it can be concluded that teeth are highly reliable and applicatable as forensic odontology for individual identification.