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Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography

 Sangwoo Park  ;  Lucy Eunju Lee  ;  Hanna Kim  ;  Ji Eun Kim  ;  Seung Jun Lee  ;  Sunggyu Yoon  ;  Seungwoo Shin  ;  Heemin Kang  ;  YongKeun Park  ;  Jason Jungsik Song  ;  Seongsoo Lee 
 SCIENTIFIC REPORTS, Vol.11(1) : 10019, 2021-05 
Journal Title
Issue Date
Optical diffraction tomography (ODT) enables imaging of unlabeled intracellular components by measuring the three-dimensional (3D) refractive index (RI). We aimed to detect intracellular monosodium urate (MSU) crystals in synovial leukocytes derived from gout patients using ODT. The 3D RI values of the synthetic MSU crystals, measured by ODT, ranged between 1.383 and 1.440. After adding synthetic MSU crystals to a macrophage, RI tomograms were reconstructed using ODT, and the reconstructed RI tomograms discerned intracellular and extracellular MSU crystals. We observed unlabeled synthetic MSU crystal entry into the cytoplasm of a macrophage through time-lapse imaging. Furthermore, using gout patient-derived synovial leukocytes, we successfully obtained RI tomogram images of intracellular MSU crystals. The 3D RI identification of MSU crystals was verified with birefringence through polarization-sensitive ODT measurements. Together, our results provide evidence that this novel ODT can identify birefringent MSU crystals in synovial leukocytes of patients with gout.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Song, Jason Jungsik(송정식) ORCID logo https://orcid.org/0000-0003-0662-7704
Lee, Seung-Jun(이승준) ORCID logo https://orcid.org/0000-0002-9201-4818
Lee, Lucy Eunju(이은주)
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