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The Dopaminergic Neuronal System Regulates the Inflammatory Status of Mouse Lacrimal Glands in Dry Eye Disease

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dc.contributor.author강현구-
dc.contributor.author김태임-
dc.contributor.author서경률-
dc.contributor.author이형근-
dc.contributor.author지용우-
dc.date.accessioned2021-05-26T16:51:30Z-
dc.date.available2021-05-26T16:51:30Z-
dc.date.issued2021-04-
dc.identifier.issn0146-0404-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/182866-
dc.description.abstractPurpose: Comparison of the parasympathetic and sympathetic neurons, including the dopaminergic neural system, in dry eye (DE)-induced pathophysiology has not been elucidated well. This study investigated the presence of dopamine receptors (DRs) and their functional roles in the lacrimal glands (LGs) of DE-induced mice. Methods: After DE was induced in B6 mice for 2 weeks, the expression of tyrosine hydroxylase (TH), dopamine, and DRs (DR1, DR2, etc.) in the LGs and corneas were measured by quantitative RT-PCR, immunoblot, and ELISA. Using flow cytometry and ELISA, immune cell infiltration and inflammatory cytokine expression were determined in DE-induced LGs with or without DR blockers, SCH-23390 (DR1i), or melperone (DR2i). Corneal erosion scores were also investigated. Results: The mRNA and protein levels of TH significantly increased in DE-induced LGs. The dopamine concentration of LGs was 9.51 pmol in DE (versus naive: 1.39 pmol; P < 0.001). Both DR1 and DR2 mRNA expression were significantly enhanced in desiccating stress compared with those in naive (3.7- and 2.1-fold, P < 0.001). Interestingly, DR1 and DR2 immunostaining patterns stained independently in DE-induced LGs. CD3+ and CD19+ cell infiltration was significantly increased by DR2i (P < 0.001) but not by DR1i. Furthermore, IFN-γ, IL-17, and TNF-α were significantly upregulated by DR2i compared with the blow-only condition. The severity of corneal erosion and inflammation was also aggravated by DR2i. Conclusions: Upregulation of DR1 and DR2 was observed in DE-induced mouse LGs. As the inflammatory conditions are aggravated by the inhibition of DRs, especially DR2, their activity may be an important factor preserving ocular surface homeostasis.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherAssociation For Research In Vision And Ophthalmology (Arvo)-
dc.relation.isPartOfINVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleThe Dopaminergic Neuronal System Regulates the Inflammatory Status of Mouse Lacrimal Glands in Dry Eye Disease-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학교실)-
dc.contributor.googleauthorYong Woo Ji-
dc.contributor.googleauthorHyun Goo Kang-
dc.contributor.googleauthorJong Suk Song-
dc.contributor.googleauthorJi Won Jun-
dc.contributor.googleauthorKyusun Han-
dc.contributor.googleauthorTae-Im Kim-
dc.contributor.googleauthorKyoung Yul Seo-
dc.contributor.googleauthorHyung Keun Lee-
dc.identifier.doi10.1167/iovs.62.4.14-
dc.contributor.localIdA04873-
dc.contributor.localIdA01080-
dc.contributor.localIdA01870-
dc.contributor.localIdA03303-
dc.contributor.localIdA03967-
dc.relation.journalcodeJ01187-
dc.identifier.eissn1552-5783-
dc.identifier.pmid33844826-
dc.contributor.alternativeNameKang, Hyun Goo-
dc.contributor.affiliatedAuthor강현구-
dc.contributor.affiliatedAuthor김태임-
dc.contributor.affiliatedAuthor서경률-
dc.contributor.affiliatedAuthor이형근-
dc.contributor.affiliatedAuthor지용우-
dc.citation.volume62-
dc.citation.number4-
dc.citation.startPage14-
dc.identifier.bibliographicCitationINVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.62(4) : 14, 2021-04-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers

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