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The Dopaminergic Neuronal System Regulates the Inflammatory Status of Mouse Lacrimal Glands in Dry Eye Disease
DC Field | Value | Language |
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dc.contributor.author | 강현구 | - |
dc.contributor.author | 김태임 | - |
dc.contributor.author | 서경률 | - |
dc.contributor.author | 이형근 | - |
dc.contributor.author | 지용우 | - |
dc.date.accessioned | 2021-05-26T16:51:30Z | - |
dc.date.available | 2021-05-26T16:51:30Z | - |
dc.date.issued | 2021-04 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/182866 | - |
dc.description.abstract | Purpose: Comparison of the parasympathetic and sympathetic neurons, including the dopaminergic neural system, in dry eye (DE)-induced pathophysiology has not been elucidated well. This study investigated the presence of dopamine receptors (DRs) and their functional roles in the lacrimal glands (LGs) of DE-induced mice. Methods: After DE was induced in B6 mice for 2 weeks, the expression of tyrosine hydroxylase (TH), dopamine, and DRs (DR1, DR2, etc.) in the LGs and corneas were measured by quantitative RT-PCR, immunoblot, and ELISA. Using flow cytometry and ELISA, immune cell infiltration and inflammatory cytokine expression were determined in DE-induced LGs with or without DR blockers, SCH-23390 (DR1i), or melperone (DR2i). Corneal erosion scores were also investigated. Results: The mRNA and protein levels of TH significantly increased in DE-induced LGs. The dopamine concentration of LGs was 9.51 pmol in DE (versus naive: 1.39 pmol; P < 0.001). Both DR1 and DR2 mRNA expression were significantly enhanced in desiccating stress compared with those in naive (3.7- and 2.1-fold, P < 0.001). Interestingly, DR1 and DR2 immunostaining patterns stained independently in DE-induced LGs. CD3+ and CD19+ cell infiltration was significantly increased by DR2i (P < 0.001) but not by DR1i. Furthermore, IFN-γ, IL-17, and TNF-α were significantly upregulated by DR2i compared with the blow-only condition. The severity of corneal erosion and inflammation was also aggravated by DR2i. Conclusions: Upregulation of DR1 and DR2 was observed in DE-induced mouse LGs. As the inflammatory conditions are aggravated by the inhibition of DRs, especially DR2, their activity may be an important factor preserving ocular surface homeostasis. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Association For Research In Vision And Ophthalmology (Arvo) | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | The Dopaminergic Neuronal System Regulates the Inflammatory Status of Mouse Lacrimal Glands in Dry Eye Disease | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학교실) | - |
dc.contributor.googleauthor | Yong Woo Ji | - |
dc.contributor.googleauthor | Hyun Goo Kang | - |
dc.contributor.googleauthor | Jong Suk Song | - |
dc.contributor.googleauthor | Ji Won Jun | - |
dc.contributor.googleauthor | Kyusun Han | - |
dc.contributor.googleauthor | Tae-Im Kim | - |
dc.contributor.googleauthor | Kyoung Yul Seo | - |
dc.contributor.googleauthor | Hyung Keun Lee | - |
dc.identifier.doi | 10.1167/iovs.62.4.14 | - |
dc.contributor.localId | A04873 | - |
dc.contributor.localId | A01080 | - |
dc.contributor.localId | A01870 | - |
dc.contributor.localId | A03303 | - |
dc.contributor.localId | A03967 | - |
dc.relation.journalcode | J01187 | - |
dc.identifier.eissn | 1552-5783 | - |
dc.identifier.pmid | 33844826 | - |
dc.contributor.alternativeName | Kang, Hyun Goo | - |
dc.contributor.affiliatedAuthor | 강현구 | - |
dc.contributor.affiliatedAuthor | 김태임 | - |
dc.contributor.affiliatedAuthor | 서경률 | - |
dc.contributor.affiliatedAuthor | 이형근 | - |
dc.contributor.affiliatedAuthor | 지용우 | - |
dc.citation.volume | 62 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 14 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.62(4) : 14, 2021-04 | - |
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