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Fabrication of Microfluidic Cell Culture Platform for Real‑time Monitoring of Lidocaine Concentration

Authors
 Hye Jin Choi  ;  Tran Bao Ngoc  ;  Hyunjik Song  ;  Chul Ho Chang  ;  Gyu Man Kim 
Citation
 INTERNATIONAL JOURNAL OF PRECISION ENGINEERING AND MANUFACTURING, Vol.21(12) : 2399-2405, 2020-10 
Journal Title
INTERNATIONAL JOURNAL OF PRECISION ENGINEERING AND MANUFACTURING
ISSN
 2234-7593 
Issue Date
2020-10
Keywords
Microfuidic cell culture platform ; Continuous monitoring ; Lidocaine ; UV-Vis spectrometry
Abstract
We describe a microfuidic cell culture platform for real-time measurement of drug concentrations during cell cultivation.
This is the frst attempt to quantify drug concentrations during cell cultivation in real-time combining ultraviolet–visible
(UV–Vis) spectroscopy with a microfuidic cell culture system in a single microfuidic chip. The microfuidic chip has two
specifc micro-chambers, one for spectroscopic measurements and the other for cell culture. NIH/3T3 fbroblast cells were
seeded and cultured in the microfuidic chip, and then the local anesthetic lidocaine was applied and its concentration was
monitored in real time. The test results showed that lidocaine negatively afected the cell growth. The lidocaine concentra tion was decreased due to its intake by cells for the frst 3 h, after which an equilibrium concentration was reached. This
is a simple but innovative method that can observe and analyze the correlation between cells and drugs in real time. The
microfuidic system presented here can be a useful tool in various felds such as in cytotoxicity and drug screening studies
Full Text
https://link.springer.com/article/10.1007%2Fs12541-020-00429-2
DOI
10.1007/s12541-020-00429-2
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Chang, Chul Ho(장철호) ORCID logo https://orcid.org/0000-0001-5647-8298
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182636
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