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Mammalian CBX7 isoforms p36 and p22 exhibit differential responses to serum, varying functions for proliferation, and distinct subcellular localization

Authors
 Kyu-Won Cho  ;  Mark Andrade  ;  Yu Zhang  ;  Young-Sup Yoon 
Citation
 SCIENTIFIC REPORTS, Vol.10(1) : 8061, 2020-05 
Journal Title
SCIENTIFIC REPORTS
Issue Date
2020-05
MeSH
Amino Acid Sequence ; Animals ; Biomarkers ; Cell Proliferation ; Fluorescent Antibody Technique ; Gene Expression Regulation* ; Humans ; Intracellular Space / metabolism ; Mice ; Polycomb Repressive Complex 1 / chemistry ; Polycomb Repressive Complex 1 / genetics* ; Polycomb Repressive Complex 1 / metabolism* ; Protein Isoforms ; Protein Transport
Abstract
CBX7 is a polycomb group protein, and despite being implicated in many diseases, its role in cell proliferation has been controversial: some groups described its pro-proliferative properties, but others illustrated its inhibitory effects on cell growth. To date, the reason for the divergent observations remains unknown. While several isoforms for CBX7 were reported, no studies investigated whether the divergent roles of CBX7 could be due to distinct functions of CBX7 isoforms. In this study, we newly identified mouse CBX7 transcript variant 1 (mCbx7v1), which is homologous to the human CBX7 gene (hCBX7v1) and is expressed in various mouse organs. We revealed that mCbx7v1 and hCBX7v1 encode a 36 kDa protein (p36CBX7) whereas mCbx7 and hCBX7v3 encode a 22 kDa protein (p22CBX7). This study further demonstrated that p36CBX7 was localized to the nucleus and endogenously expressed in proliferating cells whereas p22CBX7 was localized to the cytoplasm, induced by serum starvation in both human and mouse cells, and inhibited cell proliferation. Together, these data indicate that CBX7 isoforms are localized in different locations in a cell and play differing roles in cell proliferation. This varying function of CBX7 isoforms may help us understand the distinct function of CBX7 in various studies.
Files in This Item:
T202006302.pdf Download
DOI
10.1038/s41598-020-64908-2
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Yoon, Young Sup(윤영섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182597
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