31 60

Cited 0 times in

Priming mesenchymal stem cells with uric acid enhances neuroprotective properties in parkinsonian models

Authors
 Ha Na Kim  ;  Jin Young Shin  ;  Dong Yeol Kim  ;  Ji Eun Lee  ;  Phil Hyu Lee 
Citation
 JOURNAL OF TISSUE ENGINEERING, Vol.12 : 1-14, 2021-04 
Journal Title
 JOURNAL OF TISSUE ENGINEERING 
Issue Date
2021-04
Keywords
Parkinson’s disease ; mesenchymal stem cells ; priming ; stemness ; uric acid
Abstract
Mesenchymal stem cells (MSCs) are a potential source of cell-based disease-modifying therapy in Parkinsonian disorders. A promising approach to develop in vitro culture methods that mimic natural MSC niche is cell priming. Uric acid (UA), a powerful antioxidant, scavenges reactive oxygen species, which has a vital role in maintaining self-renewal and differentiation potential of MSCs. Here, we demonstrated that UA treatment in naïve MSCs stimulated glycolysis and upregulated transcriptional factors responsible for regulation of stemness, leading to increase in the expression levels of osteogenesis-, adipogenesis-, and chondrogenesis-related genes. UA-primed MSCs had more enhanced neuroprotective properties in cellular and parkinsonian animal models compared to naïve MSCs by inhibiting apoptotic signaling pathways. Additionally, expression of miR-137 and miR-145 was decreased in UA-treated MSCs. Our data demonstrated that priming MSCs with UA augment neuroprotective properties through enhanced self-renewal and differentiation potential, suggesting a practical strategy for improving the application of MSCs in parkinsonian disorders.
Files in This Item:
T202101313.pdf Download
DOI
10.1177/20417314211004816
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Phil Hyu(이필휴) ORCID logo https://orcid.org/0000-0001-9931-8462
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182458
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links