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Investigation of PRDM10 and PRDM13 Expression in Developing Mouse Embryos by an Optimized PACT-Based Embryo Clearing Method

Authors
 Jiwon Woo  ;  Byung-Ho Jin  ;  Mirae Lee  ;  Eunice Yoojin Lee  ;  Hyung-Seok Moon  ;  Jeong-Yoon Park  ;  Yong-Eun Cho 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.22(6) : 2892, 2021-03 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 
Issue Date
2021-03
MeSH
Animals ; Embryo, Mammalian ; Embryonic Development / genetics* ; Gene Expression Regulation, Developmental / genetics ; Histone-Lysine N-Methyltransferase / genetics* ; Imaging, Three-Dimensional / methods* ; Mice ; Transcription Factors / genetics*
Keywords
IMPACT ; PRDM10 ; PRDM13 ; embryo clearing ; passive clearing technique ; transparent embryo
Abstract
Recent developments in tissue clearing methods have significantly advanced the three-dimensional analysis of biological structures in whole, intact tissue, providing a greater understanding of spatial relationships and biological circuits. Nonetheless, studies have reported issues with maintaining structural integrity and preventing tissue disintegration, limiting the wide application of these techniques to fragile tissues such as developing embryos. Here, we present an optimized passive tissue clearing technique (PACT)-based embryo clearing method, initial embedding PACT (IMPACT)-Basic, that improves tissue rigidity without compromising optical transparency. We also present IMPACT-Advance, which is specifically optimized for thin slices of mouse embryos past E13.5. We demonstrate proof-of-concept by investigating the expression of two relatively understudied PR domain (PRDM) proteins, PRDM10 and PRDM13, in intact cleared mouse embryos at various stages of development. We observed strong PRDM10 and PRDM13 expression in the developing nervous system and skeletal cartilage, suggesting a functional role for these proteins in these tissues throughout embryogenesis.
Files in This Item:
T202101017.pdf Download
DOI
10.3390/ijms22062892
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jeong Yoon(박정윤) ORCID logo https://orcid.org/0000-0002-3728-7784
Cho, Yong Eun(조용은) ORCID logo https://orcid.org/0000-0001-9815-2720
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182329
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