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Identification of Circulating Serum miRNAs as Novel Biomarkers in Pancreatic Cancer Using a Penalized Algorithm

Authors
 Jaehoon Lee  ;  Hee Seung Lee  ;  Soo Been Park  ;  Chanyang Kim  ;  Kahee Kim  ;  Dawoon E Jung  ;  Si Young Song 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.22(3) : 1007, 2021-02 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 
Issue Date
2021-02
MeSH
Aged ; Algorithms ; Biomarkers, Tumor / blood ; Biomarkers, Tumor / genetics* ; Biomarkers, Tumor / standards ; Cholangiocarcinoma / blood ; Cholangiocarcinoma / genetics* ; Circulating MicroRNA / blood ; Circulating MicroRNA / genetics* ; Circulating MicroRNA / standards ; Female ; Humans ; Male ; Middle Aged ; Pancreatic Neoplasms / blood ; Pancreatic Neoplasms / genetics* ; Sensitivity and Specificity
Keywords
biomarker ; diagnosis ; microRNA ; pancreatic cancer ; sensitivity ; specificity
Abstract
Pancreatic cancer (PC) is difficult to detect in the early stages; thus, identifying specific and sensitive biomarkers for PC diagnosis is crucial, especially in the case of early-stage tumors. Circulating microRNAs are promising non-invasive biomarkers. Therefore, we aimed to identify non-invasive miRNA biomarkers and build a model for PC diagnosis. For the training model, blood serum samples from 63 PC patients and 63 control subjects were used. We selected 39 miRNA markers using a smoothly clipped absolute deviation-based penalized support vector machine and built a PC diagnosis model. From the double cross-validation, the average test AUC was 0.98. We validated the diagnosis model using independent samples from 25 PC patients and 81 patients with intrahepatic cholangiocarcinoma (ICC) and compared the results with those obtained from the diagnosis using carbohydrate antigen 19-9. For the markers miR-155-5p, miR-4284, miR-346, miR-7145-5p, miR-5100, miR-661, miR-22-3p, miR-4486, let-7b-5p, and miR-4703-5p, we conducted quantitative reverse transcription PCR using samples from 17 independent PC patients, 8 ICC patients, and 8 healthy individuals. Differential expression was observed in samples from PC patients. The diagnosis model based on the identified markers showed high sensitivity and specificity for PC detection and is potentially useful for early PC diagnosis.
Files in This Item:
T202100307.pdf Download
DOI
10.3390/ijms22031007
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Song, Si Young(송시영) ORCID logo https://orcid.org/0000-0002-1417-4314
Lee, Hee Seung(이희승) ORCID logo https://orcid.org/0000-0002-2825-3160
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182054
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