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Ex Vivo Live Full-Thickness Porcine Skin Model as a Versatile In Vitro Testing Method for Skin Barrier Research

Authors
 Jee-Hyun Hwang  ;  Haengdueng Jeong  ;  Nahyun Lee  ;  Sumin Hur  ;  Nakyum Lee  ;  Jeong Jun Han  ;  Hye Won Jang  ;  Wang Keun Choi  ;  Ki Taek Nam  ;  Kyung-Min Lim 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.22(2) : 657-657, 2021-01 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 
Issue Date
2021-01
MeSH
Animals ; Biomarkers ; Epidermis / drug effects ; Epidermis / metabolism ; Fluorescent Antibody Technique ; Histocytochemistry ; Humans ; Hydroxy Acids / chemistry ; Hydroxy Acids / pharmacology ; In Vitro Techniques ; Permeability ; Rhodamines / pharmacology ; Salicylic Acid / chemistry ; Salicylic Acid / pharmacology ; Skin / cytology ; Skin / drug effects ; Skin / metabolism* ; Skin Physiological Phenomena* / drug effects ; Swine ; Tissue Culture Techniques
Keywords
ex vivo skin model ; hydroxyacids ; skin barrier ; skin permeability ; stratum corneum
Abstract
Since the European Union (EU) announced their animal testing ban in 2013, all animal experiments related to cosmetics have been prohibited, creating a demand for alternatives to animal experiments for skin studies. Here, we investigated whether an ex vivo live porcine skin model can be employed to study the safety and skin barrier-improving effects of hydroxyacids widely used in cosmetics for keratolytic peels. Glycolic acid (1-10%), salicylic acid (0.2-2%), and lactobionic acid (1.2-12%) were used as representative substances for α-hydroxyacid (AHA), β-hydroxyacid (BHA), and polyhydroxyacid (PHA), respectively. When hydroxyacids were applied at high concentrations on the porcine skin every other day for 6 days, tissue viability was reduced to 50-80%, suggesting that the toxicity of cosmetic ingredients can be evaluated with this model. Based on tissue viability, the treatment scheme was changed to a single exposure for 20 min. The protective effects of a single exposure of hydroxyacids on skin barrier function were evaluated by examining rhodamine permeability and epidermal structural components of barrier function using immunohistochemistry (IHC) and immunofluorescence (IF) staining. Lactobionic acid (PHAs) improved skin barrier function most compared to other AHAs and BHAs. Most importantly, trans-epidermal water loss (TEWL), an important functional marker of skin barrier function, could be measured with this model, which confirmed the significant skin barrier-protective effects of PHAs. Collectively, we demonstrated that the ex vivo live full-thickness porcine skin model can be an excellent alternative to animal experiments for skin studies on the safety and efficacy of cosmetic ingredients.
Files in This Item:
T202100195.pdf Download
DOI
10.3390/ijms22020657
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Nam, Ki Taek(남기택)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182015
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