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Role of binding immunoglobulin protein (BiP) in Graves' orbitopathy pathogenesis

DC FieldValueLanguage
dc.contributor.authorKo, JaeSang-
dc.contributor.authorKim, Ji-Young-
dc.contributor.authorLee, Eun Jig-
dc.contributor.authorYoon, Jin Sook-
dc.date.accessioned2021-03-31T02:14:35Z-
dc.date.available2021-03-31T02:14:35Z-
dc.date.created2021-02-22-
dc.date.issued2021-01-
dc.identifier.issn0952-5041-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/181874-
dc.description.abstractInflammation and adipogenesis represent the main pathogenic mechanisms of Graves' orbitopathy (GO), and oxidative stress is a well-known inducer of GO pathology. Endoplasmic reticulum (ER) stress has been suggested as a major contributor to inflammation and reactive oxygen species (ROS) generation. In this study, we investigated the role of the ER-stress chaperone protein, binding immunoglobulin protein (BiP), in GO pathogenesis. Using primary cultures of orbital fibroblasts from patients with GO, we examined the role of BiP in GO pathogenesis by silencing its expression with small-interfering RNA (siRNA). Inflammatory cytokine expression was analysed by Western blotting and ELISA. Intracellular ROS levels induced by hydrogen peroxide or cigarette smoke extract were measured by 5-(and 6)-carboxy-20,70-dichlorodihydrofluorescein diacetate staining and flow cytometry. After adipogenic differentiation in BiP siRNA-transfected cells, the cells were stained with Oil Red O, and the levels of adipogenic transcription factors were determined by Western blot analysis. BiP mRNA expression levels were significantly higher in GO orbital tissues than in non-GO orbital tissues. Silencing BiP attenuated the expression of pro-inflammatory cytokines (interleukin-6, intercellular adhesion molecule-1, and monocyte chemotactic protein-1) in primary cultured GO orbital fibroblasts. Silencing BiP also reduced ROS generation, hyaluronan production, and adipocyte differentiation. These findings suggest that ER stress is involved in the aetiology of GO and that modulation of ER stress has therapeutic potential for GO.-
dc.language영어-
dc.publisherBIOSCIENTIFICA LTD-
dc.relation.isPartOfJOURNAL OF MOLECULAR ENDOCRINOLOGY-
dc.titleRole of binding immunoglobulin protein (BiP) in Graves' orbitopathy pathogenesis-
dc.typeArticle-
dc.contributor.googleauthorKo, JaeSang-
dc.contributor.googleauthorKim, Ji-Young-
dc.contributor.googleauthorLee, Eun Jig-
dc.contributor.googleauthorYoon, Jin Sook-
dc.identifier.doi10.1530/JME-20-0155-
dc.relation.journalcodeJ01606-
dc.identifier.eissn1479-6813-
dc.subject.keywordbinding immunoglobulin protein-
dc.subject.keywordendoplasmic reticulum stress-
dc.subject.keywordGraves&apos-
dc.subject.keywordorbitopathy-
dc.subject.keywordthyroid eye disease-
dc.subject.keywordunfolded protein response-
dc.contributor.affiliatedAuthorKo, JaeSang-
dc.contributor.affiliatedAuthorLee, Eun Jig-
dc.contributor.affiliatedAuthorYoon, Jin Sook-
dc.identifier.scopusid2-s2.0-85098941030-
dc.identifier.wosid000605294800010-
dc.citation.titleJOURNAL OF MOLECULAR ENDOCRINOLOGY-
dc.citation.volume66-
dc.citation.number1-
dc.citation.startPage71-
dc.citation.endPage81-
dc.identifier.bibliographicCitationJOURNAL OF MOLECULAR ENDOCRINOLOGY, Vol.66(1) : 71-81, 2021-01-
dc.identifier.rimsid67592-
dc.type.rimsART-
dc.description.journalClass1-
dc.description.journalClass1-
dc.subject.keywordAuthorbinding immunoglobulin protein-
dc.subject.keywordAuthorendoplasmic reticulum stress-
dc.subject.keywordAuthorGraves&apos-
dc.subject.keywordAuthororbitopathy-
dc.subject.keywordAuthorthyroid eye disease-
dc.subject.keywordAuthorunfolded protein response-
dc.subject.keywordPlusENDOPLASMIC-RETICULUM STRESS-
dc.subject.keywordPlusIN-VITRO MODEL-
dc.subject.keywordPlusTHYROID EYE DISEASE-
dc.subject.keywordPlusER STRESS-
dc.subject.keywordPlusOXIDATIVE STRESS-
dc.subject.keywordPlusORBITAL FIBROBLASTS-
dc.subject.keywordPlusRHEUMATOID-ARTHRITIS-
dc.subject.keywordPlusADIPOGENESIS-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusTISSUE-
dc.type.docTypeArticle-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalWebOfScienceCategoryEndocrinology & Metabolism-
dc.relation.journalResearchAreaEndocrinology & Metabolism-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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