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NEUROD1 Intrinsically Initiates Differentiation of Induced Pluripotent Stem Cells into Neural Progenitor Cells

Authors
 Won-Young Choi  ;  Ji-Hyun Hwang  ;  Ann-Na Cho  ;  Andrew J Lee  ;  Inkyung Jung  ;  Seung-Woo Cho  ;  Lark Kyun Kim  ;  Young-Joon Kim 
Citation
 MOLECULES AND CELLS, Vol.43(12) : 1011-1022, 2020-12 
Journal Title
 MOLECULES AND CELLS 
ISSN
 1016-8478 
Issue Date
2020-12
Keywords
Hi-C ; NEUROD1 ; chromatin accessibility ; induced pluripotent stem cell ; neuronal progenitor cel
Abstract
Cell type specification is a delicate biological event in which every step is under tight regulation. From a molecular point of view, cell fate commitment begins with chromatin alteration, which kickstarts lineage-determining factors to initiate a series of genes required for cell specification. Several important neuronal differentiation factors have been identified from ectopic over-expression studies. However, there is scarce information on which DNA regions are modified during induced pluripotent stem cell (iPSC) to neuronal progenitor cell (NPC) differentiation, the cis regulatory factors that attach to these accessible regions, or the genes that are initially expressed. In this study, we identified the DNA accessible regions of iPSCs and NPCs via the Assay for Transposase-Accessible Chromatin sequencing (ATACseq). We identified which chromatin regions were modified after neuronal differentiation and found that the enhancer regions had more active histone modification changes than the promoters. Through motif enrichment analysis, we found that NEUROD1 controls iPSC differentiation to NPC by binding to the accessible regions of enhancers in cooperation with other factors such as the Hox proteins. Finally, by using Hi-C data, we categorized the genes that directly interacted with the enhancers under the control of NEUROD1 during iPSC to NPC differentiation.
DOI
10.14348/molcells.2020.0207
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Lark Kyun(김락균) ORCID logo https://orcid.org/0000-0001-5983-4470
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/181549
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