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Pig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors

Authors
 Ji-Hong Bong  ;  Tae-Hun Kim  ;  Jaeyong Jung  ;  Soo Jeong Lee  ;  Jeong Soo Sung  ;  Chang Kyu Lee  ;  Min-Jung Kang  ;  Hyun Ok Kim  ;  Jae-Chul Pyun 
Citation
 BIOCHIP JOURNAL, Vol.14(4) : 358-368, 2020-12 
Journal Title
 BIOCHIP JOURNAL 
ISSN
 1976-0280 
Issue Date
2020-12
Keywords
Antibody ; COVID-19 ; Immunoassay ; Nucleoprotein ; SARS-CoV-2 ; Surface plasmon resonance
Abstract
Anti-coronavirusdisease-2019 (COVID-19; anti-severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2)) antibodies against nucleoprotein (NP) were purified from pig sera. Following the separation of the antibody fraction using a protein-A column, the final yield of the purified antibodies against SARS-CoV-2 NPs was estimated to be 0.26 ± 0.05 % (absolute amount of 143.4 ± 25.2 ng, n=5) from 1 mL of pig sera. The binding activities of the isolated antibodies were confirmed using immunoassay and immunostaining. Based on the specific binding activity to NPs, a quantitative assay was performed using a surface plasmon resonance (SPR) biosensor. From the doseresponse curve, the binding constant (Kd) was calculated to be 185 pM and the limit of detection was estimated to be 1.02 pM. The SPR biosensor with the isolated antibodies against SARS-CoV-2 NPs was applied for the detection of SARS-CoV-2, MERS-CoV, and CoV strain 229E in culture fluid.
Files in This Item:
T202005213.pdf Download
DOI
10.1007/s13206-020-4404-z
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/180761
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