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Pig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors

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dc.contributor.author김현옥-
dc.date.accessioned2020-12-11T08:04:06Z-
dc.date.available2020-12-11T08:04:06Z-
dc.date.issued2020-12-
dc.identifier.issn1976-0280-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/180761-
dc.description.abstractAnti-coronavirusdisease-2019 (COVID-19; anti-severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2)) antibodies against nucleoprotein (NP) were purified from pig sera. Following the separation of the antibody fraction using a protein-A column, the final yield of the purified antibodies against SARS-CoV-2 NPs was estimated to be 0.26 ± 0.05 % (absolute amount of 143.4 ± 25.2 ng, n=5) from 1 mL of pig sera. The binding activities of the isolated antibodies were confirmed using immunoassay and immunostaining. Based on the specific binding activity to NPs, a quantitative assay was performed using a surface plasmon resonance (SPR) biosensor. From the doseresponse curve, the binding constant (Kd) was calculated to be 185 pM and the limit of detection was estimated to be 1.02 pM. The SPR biosensor with the isolated antibodies against SARS-CoV-2 NPs was applied for the detection of SARS-CoV-2, MERS-CoV, and CoV strain 229E in culture fluid.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherKorean Biochip Society-
dc.relation.isPartOfBIOCHIP JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titlePig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.googleauthorJi-Hong Bong-
dc.contributor.googleauthorTae-Hun Kim-
dc.contributor.googleauthorJaeyong Jung-
dc.contributor.googleauthorSoo Jeong Lee-
dc.contributor.googleauthorJeong Soo Sung-
dc.contributor.googleauthorChang Kyu Lee-
dc.contributor.googleauthorMin-Jung Kang-
dc.contributor.googleauthorHyun Ok Kim-
dc.contributor.googleauthorJae-Chul Pyun-
dc.identifier.doi10.1007/s13206-020-4404-z-
dc.contributor.localIdA01122-
dc.relation.journalcodeJ00292-
dc.identifier.eissn2092-7843-
dc.identifier.pmid33133395-
dc.subject.keywordAntibody-
dc.subject.keywordCOVID-19-
dc.subject.keywordImmunoassay-
dc.subject.keywordNucleoprotein-
dc.subject.keywordSARS-CoV-2-
dc.subject.keywordSurface plasmon resonance-
dc.contributor.alternativeNameKim, Hyun Ok-
dc.contributor.affiliatedAuthor김현옥-
dc.citation.volume14-
dc.citation.number4-
dc.citation.startPage358-
dc.citation.endPage368-
dc.identifier.bibliographicCitationBIOCHIP JOURNAL, Vol.14(4) : 358-368, 2020-12-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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