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Wound Healing Potential of Low Temperature Plasma in Human Primary Epidermal Keratinocytes

Authors
 Hui Song Cui  ;  Yoon Soo Cho  ;  So Young Joo  ;  Chin Hee Mun  ;  Cheong Hoon Seo  ;  June-Bum Kim 
Citation
 TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol.16(6) : 585-593, 2019-09 
Journal Title
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
ISSN
 1738-2696 
Issue Date
2019-09
MeSH
Angiotensin I / genetics ; Angiotensin I / metabolism ; Cell Movement / drug effects* ; Cell Survival / drug effects ; Cells, Cultured ; Cytokines / metabolism ; Fibroblast Growth Factor 2 / genetics ; Fibroblast Growth Factor 2 / metabolism ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism ; Keratinocytes / cytology ; Keratinocytes / metabolism ; Plasma Gases / chemistry ; Plasma Gases / pharmacology* ; Temperature ; Vascular Endothelial Growth Factor A / genetics ; Vascular Endothelial Growth Factor A / metabolism ; Wound Healing / drug effects*
Keywords
Cytokine ; Growth factor ; Keratinocyte ; Low temperature plasma ; Wound healing
Abstract
Background: Low temperature plasma (LTP) was recently shown to be potentially useful for biomedical applications such as bleeding cessation, cancer treatment, and wound healing, among others. Keratinocytes are a major cell type that migrates directionally into the wound bed, and their proliferation leads to complete wound closure during the cutaneous repair/regeneration process. However, the beneficial effects of LTP on human keratinocytes have not been well studied. Therefore, we investigated migration, growth factor production, and cytokine secretion in primary human keratinocytes after LTP treatment.

Methods: Primary cultured keratinocytes were obtained from human skin biopsies. Cell viability was measured with the EZ-Cytox cell viability assay, cell migration was evaluated by an in vitro wound healing assay, gene expression was analyzed by quantitative real-time polymerase chain reaction, and protein expression was measured by enzyme-linked immunosorbent assays and western blotting after LTP treatment.

Results: Cell migration, the secretion of several cytokines, and gene and protein levels of angiogenic growth factors increased in LTP-treated human keratinocytes without associated cell toxicity. LTP treatment also significantly induced the expression of hypoxia inducible factor-1α (HIF-1α), an upstream regulator of angiogenesis. Further, the inhibition of HIF-1α expression blocked the production of angiogenic growth factors induced by LTP in human keratinocytes.

Conclusion: Our results suggest that LTP treatment is an effective approach to modulate wound healing-related molecules in epidermal keratinocytes and might promote angiogenesis, leading to improved wound healing.
Files in This Item:
T201906492.pdf Download
DOI
10.1007/s13770-019-00215-w
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Mun, Chin Hee(문진희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/180621
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