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Hyaluronic Acid/Alginate Hydrogel Containing Hepatocyte Growth Factor and Promotion of Vocal Fold Wound Healing

Authors
 Jeong-Seok Choi  ;  Se Heang Oh  ;  Young-Mo Kim  ;  Jae-Yol Lim 
Citation
 TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol.17(5) : 651-658, 2020-10 
Journal Title
 TISSUE ENGINEERING AND REGENERATIVE MEDICINE 
ISSN
 1738-2696 
Issue Date
2020-10
Keywords
Alginate ; Hepatocyte growth factor ; Hyaluronic acid ; Vocal fold ; Wound healing
Abstract
Background: Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing. This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model. Methods: HGF was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile was evaluated. In addition, HGF-HA/ALG was injected into the VFs of rabbits immediately after direct injury and HGF or PBS was injected in the same manner into control groups. Macroscopic features were observed by endoscopy at 3 months post-injury. Functional analyses including mucosal waves of VFs and viscoelastic properties were performed by kymography following high-speed digital imaging and rheometer. Histopathological and immunohistochemical evaluations were also conducted on VFs. Results: HGF release from HGF-HA/ALG was sustained for up to 3 weeks. Rabbits treated with HGF-HA/ALG showed improved mucosal vibrations and VF viscoelastic properties as compared with the PBS and HGF controls. Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury. Conclusion: HGF containing HA/ALG hydrogel enhanced healing in our rabbit model of VF injury.
Full Text
https://link.springer.com/article/10.1007/s13770-020-00280-6
DOI
10.1007/s13770-020-00280-6
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Lim, Jae Yol(임재열) ORCID logo https://orcid.org/0000-0002-9757-6414
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/180307
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