Biopsy is a critical method for disease diagnosis and treatment planning. It can be applied from simple inflammatory lesions to malignant tumors. But many general dental practitioners are unfamiliar with the basic knowledge and skills required for biopsy. Moreover, biopsy indications and contraindications for certain diseases may differ depending on the type of dental practice environment and the specialty of the dentist. Biopsy education can increase the choices a dentist has during disease diagnosis. Here we will discuss details on biopsy needed to the general dentist.