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Coffee Ring Effect Free TiO2 Nanotube Array for Quantitative Laser Desorption/Ionization Mass Spectrometry

 Moon-Ju Kim  ;  Jong-Min Park  ;  Joo-Yoon Noh  ;  Tae Gyeong Yun  ;  Min-Jung Kang  ;  Nam Su Ku  ;  Eun Hye Lee  ;  Kwang Hwan Park  ;  Moo Suk Park  ;  Sang-Guk Lee  ;  Jae-Chul Pyun 
 ACS APPLIED NANO MATERIALS, Vol.3(9) : 9249-9259, 2020-09 
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TiO2 nanotube arrays ; coffee ring effect ; wettability control ; sepsis ; quantitative LDI-MS
Quantitative analysis using laser desorption/ionization mass spectrometry (LDI-MS) has been limited because of the nonuniform distribution of analytes caused by the “coffee ring effect”. In this study, a TiO2 nanotube array with hydrophilic dot patterns surrounded by hydrophobic areas (HB/HL-patterned TiO2 NTA) is presented to distribute analytes uniformly as well as to concentrate them within a sample spot. The HB/HL-patterned TiO2 NTA is fabricated by (1) electrochemical anodization, (2) silane functionalization, and (3) hydrophilic patterning with a photomask under ultraviolet radiation. Suppression of the coffee ring effect on this wettability-controlled TiO2 NTA is experimentally elucidated in terms of a capillary penetration of analyte solution into the selectively permeable TiO2 NTA. The morphology of a dried fluorescein on the HB/HL-patterned TiO2 NTA demonstrates homogeneous fluorescein deposition without lateral spreading. The feasibility of HB/HL-patterned TiO2 NTA for quantitative LDI-MS is demonstrated using two types of sepsis biomarkers, lysophosphatidylcholine 16:0 (LPC 16:0) and LPC 18:0 as model analytes. Further, LDI-MS based on the HB/HL-patterned TiO2 NTA matrix is applied to the medical diagnosis of sepsis by analyzing LPC 16:0 and LPC 18:0 with the sera from sepsis-negative and -positive groups. These results reveal that the HB/HL-patterned TiO2 NTA can be a remarkable platform for quantitative analysis. Furthermore, LDI-MS based on the HB/HL-patterned TiO2 NTA matrix has the potential to be a practical approach for sepsis diagnosis.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Ku, Nam Su(구남수) ORCID logo https://orcid.org/0000-0002-9717-4327
Park, Kwang Hwan(박광환) ORCID logo https://orcid.org/0000-0002-2110-0559
Park, Moo Suk(박무석) ORCID logo https://orcid.org/0000-0003-0820-7615
Lee, Sang-Guk(이상국) ORCID logo https://orcid.org/0000-0003-3862-3660
Lee, Eun Hye(이은혜) ORCID logo https://orcid.org/0000-0003-2570-3442
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