Cited 3 times in
STAT3-mediated MLST8 gene expression regulates cap-dependent translation in cancer cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정호성 | - |
dc.contributor.author | 김혜영 | - |
dc.date.accessioned | 2020-09-30T16:45:59Z | - |
dc.date.available | 2020-09-30T16:45:59Z | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 1574-7891 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/179614 | - |
dc.description.abstract | Signal transducer and activator of transcription 3 (STAT3) regulates cell growth, cell survival, angiogenesis, metastasis of cancer cells, and cancer immune evasion by regulating gene expression as a transcription factor. However, the effect of STAT3 on translation is almost unknown. We demonstrated that STAT3 acts as a trans-acting factor for MLST8 gene expression and the protein level of mLST8, a core component of mechanistic target of rapamycin complex 1 and 2 (mTORC1/2), positively regulates the mTORC1/2 downstream pathways. Suppression of STAT3 by siRNA attenuated 4E-BP1 phosphorylation, cap-dependent translation, and cell proliferation in a variety of cancer cells. In HCT116 cells, STAT3 knockdown-induced decreases in 4E-BP1 and AKT phosphorylation levels were further attenuated by MLST8 knockdown or recovered by mLST8 overexpression. STAT3 knockdown-induced G2/M phase arrest was partially restored by co-knockdown of 4EBP1, and the attenuation of cell proliferation was enhanced by the expression of an mTORC1-mediated phosphorylation-defective mutant of 4E-BP1. ChIP and promoter mapping using a luciferase reporter assay showed that the -951 to -894 bp of MLST8 promoter seems to include STAT3-binding site. Overall, these results suggest that STAT3-driven MLST8 gene expression regulates cap-dependent translation through 4E-BP1 phosphorylation in cancer cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | John Wiley & Sons, Inc | - |
dc.relation.isPartOf | MOLECULAR ONCOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | STAT3-mediated MLST8 gene expression regulates cap-dependent translation in cancer cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anatomy (해부학교실) | - |
dc.contributor.googleauthor | Hyunji Lee | - |
dc.contributor.googleauthor | Hyunjung Chin | - |
dc.contributor.googleauthor | Hyeyoung Kim | - |
dc.contributor.googleauthor | Hosung Jung | - |
dc.contributor.googleauthor | Daekee Lee | - |
dc.identifier.doi | 10.1002/1878-0261.12735 | - |
dc.contributor.localId | A03786 | - |
dc.relation.journalcode | J03480 | - |
dc.identifier.eissn | 1878-0261 | - |
dc.identifier.pmid | 32495998 | - |
dc.subject.keyword | 4E-BP1 | - |
dc.subject.keyword | MLST8 | - |
dc.subject.keyword | STAT3 | - |
dc.subject.keyword | cross-talk | - |
dc.subject.keyword | mTORC1 | - |
dc.contributor.alternativeName | Jung, Ho Sung | - |
dc.contributor.affiliatedAuthor | 정호성 | - |
dc.citation.volume | 14 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1850 | - |
dc.citation.endPage | 1867 | - |
dc.identifier.bibliographicCitation | MOLECULAR ONCOLOGY, Vol.14(8) : 1850-1867, 2020-08 | - |
dc.identifier.rimsid | 67207 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.