Cited 5 times in
4-Methylumbelliferone suppresses hyaluronan and adipogenesis in primary cultured orbital fibroblasts from Graves' orbitopathy
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이은직 | - |
dc.contributor.author | 윤진숙 | - |
dc.contributor.author | 채민경 | - |
dc.date.accessioned | 2020-09-28T11:42:23Z | - |
dc.date.available | 2020-09-28T11:42:23Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 0721-832X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/179257 | - |
dc.description.abstract | Purpose: In Graves' orbitopathy (GO), hyaluronan secreted by orbital fibroblasts contributes to orbital tissue expansion. The goal of this research was to evaluate the potential benefit of 4-methylumbelliferone (4-MU), a hyaluronan synthase (HAS) inhibitor, in primary cultured orbital fibroblasts from Graves' orbitopathy. Methods: We assessed the viability of orbital fibroblasts using a live/dead cell assay. Hyaluronan synthesis was evaluated by enzyme-linked immunosorbent assay (ELISA) and quantitative real-time PCR (qPCR). Adipogenesis was assessed by Oil Red O staining and qPCR of adipogenic transcription factors. Results: In orbital fibroblasts treated with 4-MU (up to 1000 μM), cell viability was preserved by 90%. 4-MU significantly inhibited HAS gene expression and hyaluronan production (*P < 0.05). With respect to adipogenesis, 4-MU suppressed the accumulation of lipids and reduced the number of adipocytes, while decreasing expression of adipogenic transcription factors. Conclusions: 4-MU represents a promising new therapeutic agent for GO based on its ability to inhibit hyaluronan production and adipogenesis, without decreasing cell viability. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English, German | - |
dc.publisher | Springer-Verlag | - |
dc.relation.isPartOf | GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | 4-Methylumbelliferone suppresses hyaluronan and adipogenesis in primary cultured orbital fibroblasts from Graves' orbitopathy | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Yeonjung Yoon | - |
dc.contributor.googleauthor | Min Kyung Chae | - |
dc.contributor.googleauthor | Eun Jig Lee | - |
dc.contributor.googleauthor | Jin Sook Yoon | - |
dc.identifier.doi | 10.1007/s00417-019-04528-3 | - |
dc.contributor.localId | A03050 | - |
dc.contributor.localId | A02611 | - |
dc.relation.journalcode | J00951 | - |
dc.identifier.eissn | 1435-702X | - |
dc.identifier.pmid | 31900640 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs00417-019-04528-3 | - |
dc.subject.keyword | 4-methylumbelliferone | - |
dc.subject.keyword | Adipogenesis | - |
dc.subject.keyword | Graves’ orbitopathy | - |
dc.subject.keyword | Hyaluronic acid | - |
dc.contributor.alternativeName | Lee, Eun Jig | - |
dc.contributor.affiliatedAuthor | 이은직 | - |
dc.contributor.affiliatedAuthor | 윤진숙 | - |
dc.citation.volume | 258 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1095 | - |
dc.citation.endPage | 1102 | - |
dc.identifier.bibliographicCitation | GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, Vol.258(5) : 1095-1102, 2020-05 | - |
dc.identifier.rimsid | 67435 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.