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An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody
DC Field | Value | Language |
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dc.contributor.author | 장윤수 | - |
dc.date.accessioned | 2020-09-28T11:31:39Z | - |
dc.date.available | 2020-09-28T11:31:39Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/179218 | - |
dc.description.abstract | AIMP2-DX2, an exon 2-deleted splice variant of AIMP2 (aminoacyl-tRNA synthetase-interacting multifunctional protein 2), is highly expressed in lung cancer and involved in tumor progression in vivo. Oncogenic function of AIMP2-DX2 and its correlation with poor prognosis of cancer patients have been well established; however, the application of this potentially important biomarker to cancer research and diagnosis has been hampered by a lack of antibodies specific for the splice variant, possibly due to the poor immunogenicity and/or stability of AIMP2-DX2. In this study a monoclonal antibody, H5, that specifically recognizes AIMP2-DX2 and its isoforms was generated via rabbit immunization and phage display techniques, using a short peptide corresponding to the exon 1/3 junction sequence as an antigen. Furthermore, based on mutagenesis, limited cleavage, and mass spectrometry studies, it is also suggested that the endogenous isoform of AIMP2-DX2 recognized by H5 is produced by proteolytic cleavage of 33 amino acids from N-terminus and is capable of inducing cell proliferation similarly to the uncleaved protein. H5 monoclonal antibody is applicable to enzyme-linked immunosorbent assay, immunoblot, immunofluorescence, and immunohistochemistry, and expected to be a valuable tool for detecting AIMP2-DX2 with high sensitivity and specificity for research and diagnostic purposes. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | BIOMOLECULES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Dae Gyu Kim | - |
dc.contributor.googleauthor | Thi Thu Ha Nguyen | - |
dc.contributor.googleauthor | Nam Hoon Kwon | - |
dc.contributor.googleauthor | Junsik Sung | - |
dc.contributor.googleauthor | Semi Lim | - |
dc.contributor.googleauthor | Eun-Joo Kang | - |
dc.contributor.googleauthor | Jihye Lee | - |
dc.contributor.googleauthor | Woo Young Seo | - |
dc.contributor.googleauthor | Arum Kim | - |
dc.contributor.googleauthor | Yoon Soo Chang | - |
dc.contributor.googleauthor | Hyunbo Shim | - |
dc.contributor.googleauthor | Sunghoon Kim | - |
dc.identifier.doi | 10.3390/biom10060820 | - |
dc.contributor.localId | A03456 | - |
dc.relation.journalcode | J03712 | - |
dc.identifier.eissn | 2218-273X | - |
dc.identifier.pmid | 32471182 | - |
dc.subject.keyword | AIMP2-DX2 | - |
dc.subject.keyword | antibody | - |
dc.subject.keyword | diagnostic marker | - |
dc.subject.keyword | phage display | - |
dc.subject.keyword | splice variant | - |
dc.contributor.alternativeName | Chang, Yoon Soo | - |
dc.contributor.affiliatedAuthor | 장윤수 | - |
dc.citation.volume | 10 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 820 | - |
dc.identifier.bibliographicCitation | BIOMOLECULES, Vol.10(6) : 820, 2020-05 | - |
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