47 63

Cited 0 times in

In Situ Profiling of the Three Dominant Phyla Within the Human Gut Using TaqMan PCR for Pre-Hospital Diagnosis of Gut Dysbiosis

Authors
 Young Jae Jo  ;  Setu Bazie Tagele  ;  Huy Quang Pham  ;  YeonGyun Jung  ;  Jerald Conrad Ibal  ;  SeungDae Choi  ;  Gi-Ung Kang  ;  Sowon Park  ;  Yunkoo Kang  ;  Seung Kim  ;  Hong Koh  ;  Jae-Ho Shin 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.21(6) : 1916, 2020-03 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 
Issue Date
2020-03
Keywords
TaqMan probes ; gut microbiota ; next generation sequencing ; primer design ; qPCR ; ulcerative colitis
Abstract
A microbial imbalance called dysbiosis leads to inflammatory bowel disease (IBD), which can include ulcerative colitis (UC). Fecal microbiota transplantation (FMT), a novel therapy, has recently been successful in treating gut dysbiosis in UC patients. For the FMT technique to be successful, the gut microbiota of both the healthy donors and UC patients must be characterized. For decades, next-generation sequencing (NGS) has been used to analyze gut microbiota. Despite the popularity of NGS, the cost and time constraints make it difficult to use in emergency services and activities related to the periodic monitoring of microbiota profile alterations. Hence, in this study, we developed a multiplex TaqMan qPCR assay (MTq-PCR) with novel probes to simultaneously determine the relative proportions of the three dominant microbial phyla in the human gut: Bacteroidetes, Firmicutes, and Proteobacteria. The relative proportions of the three phyla in fecal samples of either healthy volunteers or UC patients were similar when assessed NGS and the MTq-PCR. Thus, our MTq-PCR assay could be a practical microbiota profiling alternative for diagnosing and monitoring gut dysbiosis in UC patients during emergency situations, and it could have a role in screening stool from potential FMT donors.
Files in This Item:
T202002256.pdf Download
DOI
10.3390/ijms21061916
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
Yonsei Authors
Koh, Hong(고홍) ORCID logo https://orcid.org/0000-0002-3660-7483
Kim, Seung(김승) ORCID logo https://orcid.org/0000-0003-4373-9828
Park, So Won(박소원) ORCID logo https://orcid.org/0000-0002-2498-8004
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/179047
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links