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Effects and regulation of osteopontin in rat hepatic stellate cells

Authors
 Sung Hee Lee  ;  Geom Seog Seo  ;  Young Nyun Park  ;  Tae Moo Yoo  ;  Dong Hwan Sohn 
Citation
 BIOCHEMICAL PHARMACOLOGY, Vol.68(12) : 2367-2378, 2004-12 
Journal Title
 BIOCHEMICAL PHARMACOLOGY 
ISSN
 0006-2952 
Issue Date
2004-12
MeSH
Animals ; Carbon Tetrachloride / toxicity ; Cell Movement / physiology ; Cell Proliferation ; Collagen Type I / metabolism ; Humans ; Kupffer Cells / metabolism* ; Liver / cytology* ; Liver Cirrhosis / chemically induced ; Liver Cirrhosis / metabolism ; Matrix Metalloproteinase 2 / metabolism ; Matrix Metalloproteinases, Membrane-Associated ; Metalloendopeptidases / metabolism ; Osteopontin ; Rats ; Rats, Sprague-Dawley ; Receptors, Transforming Growth Factor beta / metabolism ; Sialoglycoproteins / immunology ; Sialoglycoproteins / isolation & purification* ; Sialoglycoproteins / physiology ; Tissue Inhibitor of Metalloproteinase-2 / metabolism ; Wound Healing / physiology
Abstract
Using a cDNA microarray, we identified osteopontin (OPN) as one of the genes upregulated in cultured activated hepatic stellate cells (HSCs). Northern and western blot analyses showed that OPN was increasingly expressed during the progressive activation of cultured rat HSCs, and a significant increase in OPN was observed in carbon tetrachloride-induced rat liver fibrosis. In biliary atresia, OPN protein was predominantly expressed in Kupffer cells and HSCs in the necrotic areas. Incubation of HSCs with recombinant OPN-induced significant proliferative and migratory effects, and induced matrix metalloproteinase 2 production and activation. Moreover, OPN increased type I collagen production and type II transforming growth factor-beta receptor mRNA and protein. In conclusion, this study shows that OPN is expressed in activated HSCs and suggests that the upregulation of OPN might be a central pathway of HSC activation.
Full Text
https://www.sciencedirect.com/science/article/pii/S000629520400601X
DOI
10.1016/j.bcp.2004.08.022
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Nyun(박영년) ORCID logo https://orcid.org/0000-0003-0357-7967
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178789
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