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Genetic alterations in intrahepatic cholangiocarcinoma as revealed by degenerate oligonucleotide primed PCR-comparative genomic hybridization

Authors
 Ji-Young Lee  ;  Young-Nyun Park  ;  Kyung-Ok Uhm  ;  Soo-Yeun Park  ;  Sun-Hwa Park 
Citation
 JOURNAL OF KOREAN MEDICAL SCIENCE, Vol.19(5) : 682-687, 2004-10 
Journal Title
 JOURNAL OF KOREAN MEDICAL SCIENCE 
ISSN
 1011-8934 
Issue Date
2004-10
MeSH
Adult ; Aged ; Bile Duct Neoplasms / genetics* ; Bile Ducts, Intrahepatic* ; Cholangiocarcinoma / genetics* ; Chromosome Aberrations* ; DNA Primers ; Female ; Gene Dosage ; Humans ; Male ; Middle Aged ; Nucleic Acid Hybridization / methods ; Oligonucleotides ; Polymerase Chain Reaction / methods*
Abstract
Intrahepatic cholangiocarcinoma (ICC), a malignant neoplasm of the biliary epithelium,is usually fatal because of difficulty in early diagnosis and lack of availability of effective therapy. The genetic mechanisms involved in the development of ICC are not well understood and only a few cytogenetic studies of ICC have been published. Recently, technique of degenerate oligonucleotide primed (DOP)-PCR comparative genomic hybridization (CGH) permits genetic imbalances screening of the entire genome using only small amounts of tumor DNA. In this study chromosomal aberrations in 33 Korean ICC were investigated by DOP-PCR CGH. The common sites of copy number increases were 20q (67%), 17 (61%), 11q11-q13 (42%), 8p12- qter (39%), 18p (39%), 15q22-qter (36%), 16p (36%), 6p21 (30%), 3q25-qter (27%), 1q41-qter (24%), and 5p14-q11.2 (24%). DNA amplification was identified in 16 carcinomas (48%). The frequent sites of amplification were 20q, 17p, 17q23-qter, and 7p. The most frequent sites of copy number decreases were 1p32-pter (21%) and 4q (21%). The recurrent chromosomal aberrations identified in this study provide candidate regions involved in the tumorigenesis and progression of ICC.
Files in This Item:
T200404924.pdf Download
DOI
10.3346/jkms.2004.19.5.682
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Nyun(박영년) ORCID logo https://orcid.org/0000-0003-0357-7967
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178788
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