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Characterization of cis-acting elements in the rat ATP citrate-lyase gene promoter

Authors
 Young-Ah Moon  ;  Sahng-Wook Park  ;  Kyung-Sup Kim 
Citation
 EXPERIMENTAL AND MOLECULAR MEDICINE, Vol.34 : 60-68, 2002-03 
Journal Title
 EXPERIMENTAL AND MOLECULAR MEDICINE 
ISSN
 1226-3613 
Issue Date
2002-03
MeSH
Adenosine Triphosphate / metabolism* ; Animals ; Base Sequence ; CCAAT-Binding Factor / metabolism ; Cells, Cultured ; DNA Footprinting ; Dietary Carbohydrates ; Genes, Reporter ; Hepatocytes / cytology ; Molecular Sequence Data ; Multienzyme Complexes / genetics* ; Nuclear Proteins / metabolism ; Oxo-Acid-Lyases / genetics* ; Promoter Regions, Genetic* ; Rats ; Rats, Sprague-Dawley ; Transcription Factors / metabolism* ; Transcription, Genetic
Abstract
The cis-acting element in the promoter of the rat ATP-citrate lyase (ACL) and transcription factors which interact with these elements were determined. Six Sp1 binding sites and CAAT box exist in the region from transcription start site to -419 bp which showed the highest promoter activity in ACL promoter previously. In the region from -612 to -419, C/EBP binding site and other protein binding sites were also detected. Chloramphenicol acetyltransferase assay of ACL promoter suggested that multiple Sp1 sites might be involved in the ACL transcription. Gel mobility shift assay with antibodies against Sp1 and Sp3 revealed that DNA binding efficiency of Sp1 is increased in the liver of rats re-fed low fat/high carbohydrate diet after fasting. These results suggest that Sp1 is one of the most important transcription factors for ACL promoter to produce basal and induced transcription by low fat/high carbohydrate diet.
Full Text
https://www.nature.com/articles/emm20029
DOI
10.1038/emm.2002.9
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sup(김경섭) ORCID logo https://orcid.org/0000-0001-8483-8537
Park, Sahng Wook(박상욱) ORCID logo https://orcid.org/0000-0002-9594-7074
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178739
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