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위상피세포주에서 타우린 포합 담즙산에 의한 Nuclear Factor - Kappa B 매개 Interleukin - 8 의 발현
DC Field | Value | Language |
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dc.contributor.author | 김원호 | - |
dc.contributor.author | 김혜영 | - |
dc.date.accessioned | 2020-09-04T01:59:51Z | - |
dc.date.available | 2020-09-04T01:59:51Z | - |
dc.date.issued | 2002-03 | - |
dc.identifier.issn | 1598-9992 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/178738 | - |
dc.description.abstract | Background/Aims: The molecular mechanism of gastric epithelial injury induced by bile acid remains poorly understood. The aims of this study were to examine whether IL-8 was expressed by the stimulation of human gastric epithelial cells (AGS and Kato III) with taurine-conjugated bile acids, taurocholic acid (TC) or taurochenodeoxycholic acid (TCDC), and to evaluate the role of Nuclear Factor-Kappa B (NF- B) on the expression of IL-8. Methods: After the gastric epithelial cells were treated with TC or TCDC, time courses of NF- B binding activity and IL-8 secretion were determined by electrophoretic mobility shift assay (EMSA) and ELISA. To evaluate the role of NF- B on the expression of IL-8, IL-8 levels were assessed after pretreatment with rebamipide or pyrrolidine dithiocarbamate (PDTC), known as NF- B inhibitors, or phosphorothioate-modified anti-sense (AS) oligonucleotides (ODN) for p50 subunit of NF- B. Results: TC or TCDC stimulation increased IL-8 secretion in a time and dose-dependent manner. Moreover, AGS and Kato III cells treated with TC or TCDC dose-dependently induced a prominent NF- B binding complex within 60 min. Pre-incubation of the cells with PDTC (100 M), rebamipide (0.1 and 0.5 mM) or AS-ODN caused significant decreases in IL-8 secretion induced by TC or TCDC. Conclusions: NF- B mediated IL-8 expression may play an important role in the taurine-conjugated bile acid-induced gastric epithelial injury and may present a plausible molecular mechanism for the bile reflux gastritis. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean Journal of Gastroenterology | - |
dc.publisher | Korean Journal of Gastroenterology | - |
dc.relation.isPartOf | Korean Journal of Gastroenterology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | 위상피세포주에서 타우린 포합 담즙산에 의한 Nuclear Factor - Kappa B 매개 Interleukin - 8 의 발현 | - |
dc.title.alternative | Taurine - conjugated Bile Acids Induce Nuclear Factor - Kappa B Mediated Interleukin - 8 Activation in Gastric Epithelial Cell Lines. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | 백순구 | - |
dc.contributor.googleauthor | 서정인 | - |
dc.contributor.googleauthor | 김현수 | - |
dc.contributor.googleauthor | 이동기 | - |
dc.contributor.googleauthor | 권상옥 | - |
dc.contributor.googleauthor | 김혜영 | - |
dc.contributor.googleauthor | 김원호 | - |
dc.contributor.localId | A00774 | - |
dc.contributor.localId | A01175 | - |
dc.relation.journalcode | J02015 | - |
dc.identifier.eissn | 2233-6869 | - |
dc.contributor.alternativeName | Kim, Won Ho | - |
dc.contributor.affiliatedAuthor | 김원호 | - |
dc.contributor.affiliatedAuthor | 김혜영 | - |
dc.citation.volume | 39 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 204 | - |
dc.citation.endPage | 215 | - |
dc.identifier.bibliographicCitation | Korean Journal of Gastroenterology, Vol.39(3) : 204-215, 2002-03 | - |
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