Toxicologic Screening of Agmatine Using In Vitro and In Vivo Assays

Abstract

ObjectiveaaTo evaluate the both in vitro and in vivo toxicological effects of agmatine. MethodsaaThe toxicity of agmatine (50, 100 and 200 μM) was determined by using Alamar Blue assay in murine brain endothelial (bEnd.3) cells as vitro model and chick chorioallantoic membrane (CAM) assay as an in vivo model. ResultsaaThe treatment of agmatine (50, 100 and 200 μM) showed no significant increase/decrease in the total cell number compared to control in the bEnd.3 cells in in vitro. The treatment of agmatine at three different concentrations (50, 100 and 200 μM) onto the CAM tissue for 2 days, showed similar survival rates of chick embryo to that of the control group. ConclusionsaaThe present results demonstrated that agmatine (50, 100 and 200 μM) doesn’t exert any toxic effect as evidenced by the above results depicting the therapeutic potential of agmatine via modulation of vascularization for the treatment of neural diseases.