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Snail-induced EMT promotes cancer stem cell-like properties in head and neck cancer cells

 Ichiro Ota  ;  Takashi Masui  ;  Miyako Kurihara  ;  Jong-In Yook  ;  Shinji Mikami  ;  Takahiro Kimura  ;  Keiji Shimada  ;  Noboru Konishi  ;  Katsunari Yane  ;  Toshiaki Yamanaka  ;  Tadashi Kitahara 
 ONCOLOGY REPORTS, Vol.35(1) : 261-266, 2016-01 
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Carcinoma, Squamous Cell / genetics ; Carcinoma, Squamous Cell / metabolism ; Carcinoma, Squamous Cell / pathology* ; Cell Line, Tumor ; Cisplatin / pharmacology ; Drug Resistance, Neoplasm ; Epithelial-Mesenchymal Transition* ; Gene Expression Regulation, Neoplastic ; Head and Neck Neoplasms / genetics ; Head and Neck Neoplasms / metabolism ; Head and Neck Neoplasms / pathology* ; Humans ; Neoplastic Stem Cells / metabolism ; Neoplastic Stem Cells / pathology* ; Snail Family Transcription Factors ; Squamous Cell Carcinoma of Head and Neck
Epithelial-mesenchymal transition (EMT) is a key process involved in the invasion and metastasis of cancer cells. Furthermore, EMT can induce a cancer stem cell (CSC)-like phenotype in a number of tumor types. We demonstrated that Snail is one of the master regulators that promotes EMT and mediates cancer cell migration and invasion in many types of malignancies including head and neck squamous cell carcinoma (HNSCC). In the present study, we investigated the role of Snail in inducing and maintaining CSC-like properties through EMT in HNSCC. We established HNSCC cell lines transfected with Snail. Stem cell markers were evaluated with real-time RT-PCR and western blot analysis. CSC properties were assessed using sphere formation and WST-8 assays as well as chemosensitivity and chick chorioallantoic membrane in vivo invasion assays. Introduction of Snail induced EMT properties in HNSCC cells. Moreover, Snail-induced EMT maintained the CSC-like phenotype, and enhanced sphere formation capability, chemoresistance and invasive ability. These data suggest that Snail could be one of the critical molecular targets for the development of therapeutic strategies for HNSCC.
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2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Yook, Jong In(육종인) ORCID logo https://orcid.org/0000-0002-7318-6112
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