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Snail-induced epithelial-mesenchymal transition promotes cancer stem cell-like phenotype in head and neck cancer cells

Authors
 Takashi Masui  ;  Ichiro Ota  ;  Jong-In Yook  ;  Shinji Mikami  ;  Katsunari Yane  ;  Toshiaki Yamanaka  ;  Hiroshi Hosoi 
Citation
 INTERNATIONAL JOURNAL OF ONCOLOGY, Vol.44(3) : 693-699, 2014-03 
Journal Title
 INTERNATIONAL JOURNAL OF ONCOLOGY 
ISSN
 1019-6439 
Issue Date
2014-03
MeSH
Aldehyde Dehydrogenase 1 ; Cadherins / biosynthesis ; Cell Line, Tumor ; Cisplatin / administration & dosage ; Epithelial-Mesenchymal Transition / genetics* ; Gene Expression Regulation, Neoplastic / drug effects ; Head and Neck Neoplasms / genetics* ; Head and Neck Neoplasms / pathology ; Humans ; Hyaluronan Receptors / biosynthesis ; Isoenzymes / biosynthesis ; Neoplastic Stem Cells / metabolism* ; Retinal Dehydrogenase / biosynthesis ; Snail Family Transcription Factors ; Transcription Factors / genetics*
Abstract
Head and neck squamous cell carcinoma (HNSCC) is known to have a poor prognosis. The resistance to treatment and distant metastasis are important clinical problems in HNSCC. The epithelial-mesenchymal transition (EMT) is a key process in successful execution of many steps such as the invasion and metastasis for cancer cells. Snail is one of the master regulators that promote EMT in many types of malignancies including HNSCC. Recently, it has been shown that Snail-induced EMT could induce a cancer stem cell (CSC)‑like phenotype in a number of tumor types. In this study, we investigated the role of Snail in inducing EMT properties and CSC-like phenotype in HNSCC. We established HNSCC cell lines transfected with Snail. E-cadherin was analyzed using western blot analysis and immunofluorescence staining. Cell migration and invasion were assessed using wound-healing assay and modified Boyden chamber assay, respectively. CSC markers of HNSCC, CD44 and aldehyde dehydrogenase 1 (ALDH1), were also evaluated with western blot analysis, and chemosensitivity was assessed with WST-8 assay. Introduction of Snail induced EMT properties in HNSCC cells and enhanced cell migration and invasion. Moreover, Snail-induced EMT gained CSC-like phenotype and was associated with increased chemoresistance. These results suggest that Snail could be one of the attractive targets for the development of therapeutic strategies in HNSCC.
Full Text
https://www.spandidos-publications.com/ijo/44/3/693
DOI
10.3892/ijo.2013.2225
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Yook, Jong In(육종인) ORCID logo https://orcid.org/0000-0002-7318-6112
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178476
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