0 81

Cited 0 times in

Phosphorylation of Tegument Protein pp28 Contributes to Trafficking to the Assembly Compartment in Human Cytomegalovirus Infection

Authors
 Jun-Young Seo  ;  Jin Ah Heo  ;  William J Britt 
Citation
 JOURNAL OF MICROBIOLOGY, Vol.58(7) : 624-631, 2020-07 
Journal Title
 JOURNAL OF MICROBIOLOGY 
ISSN
 1225-8873 
Issue Date
2020-07
Keywords
ERGIC ; assembly compartment ; human cytomegalovirus ; phosphoprotein ; pp28 tegument protein ; trafficking
Abstract
Human cytomegalovirus (HCMV) UL99 encodes a late tegument protein pp28 that is essential for envelopment and production of infectious virus. This protein is localized to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) in transfected cells but it localizes to the cytoplasmic assembly compartment (AC) in HCMV-infected cells. Trafficking of pp28 to the AC is required for the assembly of infectious virus. The N-terminal domain (aa 1-61) of pp28 is sufficient for trafficking and function of the wild type protein during viral infection. However, residues required for authentic pp28 trafficking with the exception of the acidic cluster in the N-terminal domain of pp28 remain undefined. Monitoring protein migration on SDS-PAGE, we found that pp28 is phosphorylated in the virus-infected cells and dephosphorylated in the viral particles. By generating substitution mutants of pp28, we showed that three serine residues (aa 41-43) and a tyrosine residue (aa 34) account for its phosphorylation. The mutant forms of pp28 were localized to the plasma membrane as well as the ERGIC in transfected cells. Likewise, these mutant proteins were localized to the plasma membrane as well as the AC in virus-infected cells. These results suggested that phosphorylation of pp28 contributes to its intracellular trafficking and efficient viral assembly and incorporation.
Full Text
https://link.springer.com/article/10.1007%2Fs12275-020-0263-5
DOI
10.1007/s12275-020-0263-5
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Seo, Jun Young(서준영) ORCID logo https://orcid.org/0000-0003-4004-2013
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178134
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links