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Dermatophagoides pteronyssinus 항원 분획별 알레르기 면역반응

Other Titles
 Allergic immune responses to the fractionated antigen of Dermatophagoides pteronyssinus 
 박중원  ;  박상욱  ;  고시환  ;  김철우  ;  이한일  ;  오상환  ;  홍천수 
 Journal of Asthma, Allergy and Clinical Immunology (천식 및 알레르기), Vol.17(2) : 151-164, 1997 
Journal Title
Journal of Asthma, Allergy and Clinical Immunology(천식 및 알레르기)
Issue Date
As molecular size of the most major allergens of house dust mite(HDM) are known to be less than 30 kD, allergic immune reactions may be different according to the molecular fractions of HDM allergens. In this study, we fractionated D. pteronyssinus(D. pt) antigen according to its molecular weights,' fraction I(FI), II(FII, more than 30 kD), III(FIII, less than 30 kD), and evaluated the differences of allergic immune responses in 40 atopic patients. FIII(A/H ratio 2.44+-0.61) elicited stronger response on intradermal skin test than FI (0.76+0.10, p<0.001) and FII(0.88k0.12, p <0.001). The immunoblot analysis revealed that the nine out of 10 atopics had IgE binding bands with 15 kD protein of FIII which were not found in FI and FII. The geometric mean of absorben value of specific IgE to FIII was 0.067, which was higher than that of FII (0.013, p<0.001), with no difference from that of FI(0.042). The skin reactivity to whole D. pt antigen correlated with FIII specific IgE(r=0.3862, p<0.01), not with FI (r=0. 2605, p=0.07) and FII specific IgE(r=0. 1103, p=0.45). FI specific IgG levels in atopic sera(n=40) were significantly higher than that of FII and FIII in all IgG subclasses (p<0.001). Both atopic(n=4) and nonatopic (n=4) groups showed multiple IgG, IgG1, and IgG2 binding ba,nds at high molecular proteins in immunoblot analysis. The prevalence and density of the IgG and IgG1 binding bands to 15 kD were higher and more intense in atopics than those of nonatopics. In conclusion, we suggest that fractionated antigens of D. pteronyssinus had different antigenicities and that the allergic irnmune responses to HDMs in atopics and nonatopic are different according to molecular fractions of HDM.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Sahng Wook(박상욱) ORCID logo https://orcid.org/0000-0002-9594-7074
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Ree, Han Il(이한일)
Hong, Chein Soo(홍천수)
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