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말초 조혈모세포 채집을 위한 대용량 백혈구분반술 -두 혈액성분 채집기의 비교-

Other Titles
 Large-Volume Leukapheresis for Collection of Peripheral Blood Stem Cells - A Comparison of Two Continous Flow Cell Separators - 
Authors
 남정현  ;  임환섭  ;  조성란  ;  김현옥  ;  권오헌  ;  민유홍 
Citation
 Korean Journal of Hematology (대한혈액학회지), Vol.32(1) : 57-66, 1997 
Journal Title
 Korean Journal of Hematology (대한혈액학회지) 
ISSN
 1225-0546 
Issue Date
1997
Abstract
Backgrond : Mobilized peripheral blood stem cells(PBSCs) are now used increasingly in patients with hematologic and solid tumors to reconstitute hematopoiesis after dose-intensive chemotherapy. We evaluated the efficacy of large-volume leukapheresis(LVL) and compared the ability of Fenwal CS3000 Plus and Cobe Spectra to collect mononuclear cells(MNCs) for PBSCT. Methods: Twenty liters of whole blood per LVL were processed in 22 patients with acute leukemia and lymphoma. LVL were performed in rapid recovery phase (white cells >3×10 9/L, or CD34+ cells > 1% of white cells) after chemotherapy followed by granulocyte-colony stimulating factor. The end point of LVL was mononuclear cells (MNCs) >8×108/㎏, or CD34+ cells>6×106/kg A-35 collection chamber was used in Fenwal CS3000 Plus and whole blood flow was set at 85mL/min, whole blood to anticoagulant ratio 11∼ 13:1, interface offset 150. MNC procedure was used in Cobe Spectra, whole blood flow was 90∼100mL/min, whole blood to anticoagulant ratio 24:1 with heparin to anticoagulant and poduct bags, collection rate 1mL/min, and hematocrit 2∼3%. Results: Total 53 LVL(35 with Fenwal CS3000 Plus and 18 with Cobe Spectra) were performed on 22 patients. An average of 2.4 LVL per patient(range 1∼4) were performed. With Fenwal CS3000 Plus, post-LVL values of hematocrit, platelets and MNCs were reduced by 12.4%, 53.1%, and 33.0% and with Cobe Spectra, 9.2%, 36.1%, and 39.6%, respectively. Mean collection volume of Fenwal CS3000 Plus and Cobe Spectra were 135.7mL and 175.2mL per LVL, respectively. There was no statistical significant difference in the yields of LVL between Fenwal CS3000 Plus(3.4±1.9×108/kg MNCs, 7.2±11.2×10 6 /kg CD34+ cells)and Spectra(4.7±2.1× 10 8/kg MNCs, 7.4±9.6×106/kg CD34+ cells). The yields of LVL were correlated well with patients’ pre-MNC counts in both cell separators. Mean percentages of MNC were 95.4% with Fenwal CS3000 Plus and 74.0% with Cobe Spectra (P<0.001) and collection efficiencies were 53.6±18.8% and 57.3± 27.8%, respectively(P>0.05). LVL product with Cobe Spectra contained less red cells(10.5±2.7mL) than Fenwal CS3000 Plus(34.1±10.8mL)(P<0.001). Platelet contamination was not different for Fenwal CS3000 Plus(2.3±2.1×10 11) and Cobe Spectra(3.1±1.0×1011). Conclusion: LVL could be conveniently used for PBSC collection with good collection efficiency and safety without serious citrate toxicities. LVL products with Fenwal CS3000 Plus showed less collection volume and granulocyte contamination. The products with Cobe Spectra showed less red cell contamination and less decrease in patients’ platelet count.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
Min, Yoo Hong(민유홍) ORCID logo https://orcid.org/0000-0001-8542-9583
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177947
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