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주사전자현미경을 이용한 피부사상균의 형태학적 관찰

Other Titles
 Scanning Electron Microscopic Examination of Dermatophytes 
Authors
 윤경한  ;  정태석  ;  신항계  ;  방동식  ;  이광훈 
Citation
 Korean Journal of Medical Mycology (대한의진균학회지), Vol.2(2) : 153-160, 1997 
Journal Title
 Korean Journal of Medical Mycology (대한의진균학회지) 
ISSN
 1226-4709 
Issue Date
1997
Abstract
BACKGROUND: It is important to understand the morphologic characteristics for the identification of a microorganism. Electron microscopy has contributed a great deal to the field of mycology. Scanning electron microscope (SEM) has become a useful tool to study three-dimensional images of fungi. Vacuum dessicator aqueous fixation is a useful sample preparation technique for SEM examination of fungal specimens without surface distortions. OBJECTIVE: This study was done to observe the three-dimensional structures of dermatophytes preserving their natural growth characteristics. METHODS: Trichophyton rubrum, T. mentagrophytes, Microsporum canis, M. gypseum, and Epidermophyton floccosum were isolated and cultured on Sabourd agar, corn meal agar, or rice agar plate. Vacuum dessicator aqueous fixation was utilized as p preparation technique for SEM examinations of fungi. RESULTS: Vacuum dessicator aqueous fixation led us to examine the aerial mycelia including conidia of the dermatophytes under SEM. The five species of dermatophytes showed their own characteristic structures especially on microconidia and macroconidia. CONCLUSION: The use of ultrastructural research has made it possible to identify the species of fungi and can be expanded and improved along with the development of SEM and specimen preparation techniques.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Bang, Dong Sik(방동식)
Lee, Kwang Hoon(이광훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177808
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