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Polymerase Chain Reaction을 이용한 국내 Bacteroides fragilis 분리균에서의 장독소 유전자 검출

Other Titles
 The Detection of Enterotoxin Gene from Bacteroides fragilis Isolates in Korea by Polymerase Chain Reaction 
Authors
 정경태  ;  성원근  ;  신경섭  ;  이경원  ;  오희복 
Citation
 Journal of the Korean Society for Microbiology (대한미생물학회지), Vol.32(5) : 521-528, 1997 
Journal Title
 Journal of the Korean Society for Microbiology (대한미생물학회지) 
ISSN
 0253-3162 
Issue Date
1997
Keywords
Enterotoxigenic Bacteroides fragilis (ETBF) ; PCR ; Enterotoxin Gene
Abstract
Bacteroides fragilis is a Gram negative nonsporulating anaerobic rod bacterium that makes up about 1 to 2% of the normal human colonic microflora. In 1984, Myer et al. reported that some strains of B. fragilis produce enterotoxin and cause diarrheal disease in cattle and human. Since then it has been termed enterotoxigenic B. fragilis (ETBF). In this study, we tried to detect enterotoxin gene from 37 B. fragilis strains, isolated in Korean patients, to confirm the existence of ETBF and usefulness of PCR as a rapid diagnosis method. By this method, we identified 9 ETBF strains and confirmed their pathogenesis by cytotoxicity test. No significant cross-reactivity with other anaerobes or aerobes was observed. Thus, the PCR method may be considered useful for the sensitive and rapid detection of anaerobic infections. And the entire amplified PCR mixture was ligated into a pT7Blue T-vector and transformed into E. coli. When the nucleotide sequences of cloned PCR products were compared with reported enterotoxin gene, pBF529 inserted DNA sequence was nearly in good agreement with it but pBF570 inserted DNA sequence showed some difference at nucleotide 270-300. A search for nucleotide sequence homologies revealed that pBF529 exhibited 99%, but pBF570 indicated only 90% identity with reported enterotoxin gene. According to these results, it was suggested that ETBF toxin can be differentiated into at least 2 subtypes.
Full Text
http://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE01489657&language=ko_KR
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177589
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