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The effect of anti-hypertensive drugs on DNA synthesis and proliferation of ultured rat aortic smooth muscle cells

 Shin Wook Kang  ;  In Hee Lee  ;  Kyu Hun Choi  ;  Ho Yung Lee  ;  Dae Suk Han 
 YONSEI MEDICAL JOURNAL, Vol.38(3) : 160-166, 1997 
Journal Title
Issue Date
Animals ; Antihypertensive Agents/pharmacology* ; Aorta/drug effects* ; Aorta/metabolism* ; Cell Division/drug effects ; Cells, Cultured ; DNA/biosynthesis* ; Male ; Muscle, Smooth, Vascular/cytology ; Muscle, Smooth, Vascular/drug effects* ; Muscle, Smooth, Vascular/metabolism* ; Rats ; Rats, Sprague-Dawley
The aim of this study was to elucidate the effects of anti-hypertensive drugs, nifedipine, furosemide, hydrochlorothiazide, captopril, and atenolol on DNA synthesis and proliferation of cultured rat aortic smooth muscle cells induced by fetal calf serum. Aortic smooth muscle cells from Sprague-Dawley rats were isolated, cultured, and seeded in multi-well plates. When confluent, cells were cultured in a conditioned medium without fetal calf serum. After 72 hours, cells were cultured in the medium retaining 10% fetal calf serum with or without anti-hypertensive drugs by increasing the concentration between 10(-8) and 10(-4) M. DNA synthesis was assessed by [3H]-thymidine uptake and proliferation by cell numbers using a hemocytometer. Nifedipine at a concentration of 10(-5) M and 5 x 10(-5) M inhibited serum-induced DNA synthesis significantly by 50.8% and 86.6%, respectively (p < 0.05). The results of cell numbers paralleled those of 3H-thymidine incorporation. Serum-induced DNA synthesis was also reduced by 32.6% at the highest dose of furosemide (10(-4) M), but there was no statistical significance. Hydrochlorothiazide, captopril, and atenolol did not show anti-proliferative effect throughout any of the doses. In conclusion, among the various anti-hypertensive drugs, nifedipine seems to be most beneficial in view of its direct inhibitory effect on DNA synthesis and proliferation of smooth muscle cells, as well as for its anti-hypertensive effect.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Shin Wook(강신욱) ORCID logo https://orcid.org/0000-0002-5677-4756
Han, Dae Suk(한대석)
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