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Cytokine Genes are Down-Regulated When Attachment of Entamoeba histolytica to HT-29 Colon Epithelial Cells is Prevented

Authors
 J. M. KIM  ;  H. C. JUNG  ;  D. Z. JIN  ;  K. I. IM  ;  I. S. SONG  ;  C. Y. KIM 
Citation
 SCANDINAVIAN JOURNAL OF IMMUNOLOGY, Vol.45(6) : 613-617, 1997 
Journal Title
 SCANDINAVIAN JOURNAL OF IMMUNOLOGY 
ISSN
 0300-9475 
Issue Date
1997
MeSH
Animals ; Carbohydrates/pharmacology ; Cell Adhesion/drug effects ; Cell Communication/immunology ; Cell Count ; Cytokines/biosynthesis* ; Cytokines/drug effects ; Cytokines/genetics* ; Down-Regulation/genetics ; Down-Regulation/immunology* ; Entamoeba histolytica/growth & development ; Entamoeba histolytica/immunology ; Entamoeba histolytica/physiology* ; HT29 Cells ; Humans ; Lectins/pharmacology ; RNA, Messenger/biosynthesis
Abstract
Entamoeba histolytica can cause invasive disease by disruption of the intestinal barriers and subsequent lysis of the intestinal cells. Adherence to and contact dependent killing of host cells requires the galactose inhibitable lectin. To elucidate the mechanism whereby E. histolytica influences host defence, the authors assessed the change of proinflammatory cytokine genes expressed by colon epithelial cells in response to co-culture with E. histolytica trophozoites and carbohydrates, including galactose, N-acetyl-galactosamine or N-acetyl-lactosamine, which prevented E. histolytica from attaching to epithelial cells. After HT-29 human colon epithelial cells were co-cultured with E. histolytica trophozoites in the presence or absence of carbohydrates (0.1-100 mM), RNA was extracted from the epithelial cells by an acid guanidinium thiocyanate-phenol-chloroform method. Cytokine gene expression was assessed by quantitative RT-PCR using a synthetic internal standard, and proteins were determined by ELISA. IL-8 mRNA expressed by HT-29 cells in response to E. histolytica trophozoites was downregulated in the presence of galactose, N-acetylgalactosamine or N-acetyl-lactosamine (0.1-100 mM), and this was paralleled by decreased IL-8 protein secretion. GM-CSF and IL-1 alpha/beta mRNAs were also downregulated in those cells in the presence of these agents. These results suggest that the expression of proinflammatory cytokine genes could be inhibited by preventing E. histolytica from attaching to the host's colon epithelial cells.
DOI
10.1046/j.1365-3083.1997.d01-442.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Im, Kyung Il(임경일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177404
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