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The Activation of Na+-K+-2Cl- Cotransporter in Response to the Hypertonic Stress and Muscarinic Stimulation in Rat Parotid Acini
DC Field | Value | Language |
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dc.contributor.author | 서정택 | - |
dc.date.accessioned | 2020-07-03T17:09:25Z | - |
dc.date.available | 2020-07-03T17:09:25Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/177310 | - |
dc.description.abstract | Na^(+)-K^(+)-2Cl^(-) cotransporter has been shown to be activated in response to volume shrinkage in a number of cell types. In this study, we investigated the role of cell shrinkage in the activation of Na^(+)-K^(+)-2Cl^(-) cotransporter in rat parotid acinar cells during hypertonic stress and muscarinic stimulation. We measured the recovery rate of the intracellular pH(pH_1) from NH_4^(+)-induced alkaline load in 2', 7'bis(2-carboxy-ethyl)-5(6')-carboxyfluorescein (BCECF)-loaded cells as an index of the activity of Na^(+)-K^(+)-2Cl^(-) contransporter. The rate of pH_1 recovery was enhanced by cell shrinkage caused by a 20% increase in perfusate osmolarity, This recovery was markedly inhibited by pretreatment with bumetanide, a blocker of Na^(+)-K^(+)-2Cl^(-) cotransporter. The rate of pHi recovery was also enhanced by 10μM carbachol stimulation which caused comparable shrinkage. Muscarinic stimulation induced an increase in [Ca^2+]_1, whereas hypertonic shrinkage failed to elevate [Ca2+]_1, Therefore, Ca^2+ does not seem to be the universal messenger for the activation of Na^(+)-K^(+)-2Cl^(-) cotransporter. In addition, the prevention of cell shrinkage during carbachol stimulation by reducing the perfusate osmolarity did not inhibit the activation of the Na^(+)-K^(+)-2Cl^(-) cotransporter. These results suggest that volume shrinkage alone is not sufficient to account for the marked activation of the Na^(+)-K^(+)-2Cl^(-) cotransporter observed during muscarinic stimulation. Other components of the intracellular signalling pathway are likely to be of great significance. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Harn Rim Won Printing Co. | - |
dc.relation.isPartOf | International Journal of Oral Biology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | The Activation of Na+-K+-2Cl- Cotransporter in Response to the Hypertonic Stress and Muscarinic Stimulation in Rat Parotid Acini | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학교실) | - |
dc.contributor.googleauthor | Soo Yeon Lee | - |
dc.contributor.googleauthor | Jeong Taeg Seo | - |
dc.contributor.googleauthor | Syng-Ill Lee | - |
dc.contributor.localId | A01905 | - |
dc.relation.journalcode | J01144 | - |
dc.contributor.alternativeName | Seo, Jeong Taeg | - |
dc.contributor.affiliatedAuthor | 서정택 | - |
dc.citation.volume | 22 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 199 | - |
dc.citation.endPage | 204 | - |
dc.identifier.bibliographicCitation | International Journal of Oral Biology, Vol.22(3) : 199-204, 1997 | - |
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