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Correlation between G1 cyclins and HPV in the uterine cervix

Authors
 Nam Hoon Cho  ;  Young Tae Kim  ;  Jae Wook Kim 
Citation
 INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY, Vol.16(4) : 339-347, 1997 
Journal Title
 INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY 
ISSN
 0277-1691 
Issue Date
1997
MeSH
Adenocarcinoma/metabolism ; Adenocarcinoma/virology ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/virology ; Cervical Intraepithelial Neoplasia/metabolism ; Cervical Intraepithelial Neoplasia/virology ; Cervix Uteri/metabolism* ; Cervix Uteri/virology* ; Cyclin D1/metabolism ; Cyclin E/metabolism ; Cyclin G ; Cyclin G1 ; Cyclins/metabolism* ; Female ; Humans ; Immunohistochemistry ; Papillomaviridae/isolation & purification* ; Polymerase Chain Reaction ; Uterine Cervical Neoplasms/metabolism ; Uterine Cervical Neoplasms/virology
Abstract
G1 cyclin, as a candidate proto-oncogene, plays a key role as a cellular regulator through direct interaction with retinoblastoma gene product (pRB), p107, and cyclin-dependent kinase. Human papillomavirus (HPV) 16/18 E6/7 proteins may bind the unphosphorylated pRB and act as a down-regulator of cyclin D. However, this theory has not been proven and the relationship between cyclin E and HPV also remains unclear. Using formalin-fixed and paraffin-embedded cervical tissues, we examined for HPV types 16 and 18 by nested polymerase chain reaction (PCR), and for the accumulation of cyclin D1 and E by immunohistochemistry in 22 cases of normal cervix, 23 cases of cervical intraepithelial neoplasia (CIN), 39 cases of invasive squamous cell carcinoma (SCC), and 6 cases of adenocarcinoma. Cyclin index (CI) was defined as the percentage of positively labeled nuclei per 1000 cells. The accumulation of cyclin D1 was detected in the normal basal and parabasal epithelium and was markedly decreased in neoplastic epithelium (0.87% CI in CIN, 5.88% in SCC). However, cyclin E was absent in the normal cervix but increased in neoplastic epithelium (7.17% CI in CIN, 10.32% in SCC). Cyclin D1 expression was significantly low (p = 0.004), but cyclin E expression was significantly high (p = 0.026) in HPV-positive cases. Cyclin E plays a leading role in neoplastic transformation whereas cyclin D is down-regulated, especially when associated with HPV.
Full Text
https://ovidsp.dc2.ovid.com/sp-4.03.0b/ovidweb.cgi?WebLinkFrameset=1&S=JPIOFPFPKPEBCOEHIPBKHFPELFPBAA00&returnUrl=ovidweb.cgi%3fMain%2bSearch%2bPage%3d1%26S%3dJPIOFPFPKPEBCOEHIPBKHFPELFPBAA00&fromjumpstart=1&directlink=https%3a%2f%2fovidsp.dc2.ovid.com%2fovftpdfs%2fFPEBIPPEHFEHKP00%2ffs047%2fovft%2flive%2fgv039%2f00004347%2f00004347-199710000-00008.pdf&filename=Correlation+between+G1+Cyclins+and+HPV+in+the+Uterine+Cervix.&navigation_links=NavLinks.S.sh.22.1&link_from=S.sh.22%7c1&pdf_key=FPEBIPPEHFEHKP00&pdf_index=/fs047/ovft/live/gv039/00004347/00004347-199710000-00008&D=ovft&link_set=S.sh.22
DOI
10.1097/00004347-199710000-00008
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Young Tae(김영태) ORCID logo https://orcid.org/0000-0002-7347-1052
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177308
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