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A mechanism of differential expression of GLUT2 in hepatocyte and pancreatic β-cell line

Authors
 Jae-Woo Kim  ;  Yu-Kyong Kim  ;  Yong-Ho Ahn 
Citation
 EXPERIMENTAL AND MOLECULAR MEDICINE, Vol.30(1) : 15-20, 1998 
Journal Title
 EXPERIMENTAL AND MOLECULAR MEDICINE 
ISSN
 1226-3613 
Issue Date
1998
MeSH
Animals ; Binding Sites ; Cell Line ; DNA Footprinting ; Deoxyribonuclease I ; Gene Expression Regulation ; Glucose Transporter Type 2 ; Islets of Langerhans/cytology ; Islets of Langerhans/metabolism* ; Liver/cytology ; Liver/metabolism* ; Monosaccharide Transport Proteins/biosynthesis* ; Monosaccharide Transport Proteins/genetics ; Promoter Regions, Genetic* ; Protein Binding ; Rats ; Transcription Factor AP-1
Abstract
DNase I footprinting assay using liver nuclear extracts revealed six protected regions between nucleotide -600 and +110 and hence named Box I-VI. Upstream promoter element (UPE), a DNA element playing crucial role in transcriptional control of the tissue specific expression of pancreatic β-cell, has been detected within the proximal region of rat GLUT2 promoter. This region is included in Box VI. The protein-DNA interaction in this region (Box VI) was confirmed by mobility shift assay using liver nuclear extracts. Deletion of the region between -585 bp and -146 bp resulted in dramatic changes in promoter activity when they were expressed in liver and β-cell derived cell line. When -585/-146 construct was expressed in liver, the activity was decreased to 46%, whereas the activity in β-cell line, HIT-T15 cell, was increased by 84% when compared to -146/+190 construct. These opposing phenomena can be explained by the fact that β-cell specifically expresses the UPE binding protein. Assuming that there may be Box VI-binding protein playing negative roles both in hepatocyte and β-cell, and that the protein acts as a negative regulator of GLUT2 gene, the UPE binding protein in the β-cell may overcome the inhibition by binding to the protein.
Full Text
http://www.nature.com/emm/journal/v30/n1/abs/emm19982a.html
DOI
10.1038/emm.1998.2
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jae Woo(김재우) ORCID logo https://orcid.org/0000-0001-5456-9495
Ahn, Yong Ho(안용호) ORCID logo https://orcid.org/0000-0002-4133-0757
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177148
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